A1 Journal article (refereed)
Can Indirect Herbicide Exposure Modify the Response of the Colorado Potato Beetle to an Organophosphate Insecticide? (2019)

Margus, A., Rainio, M., & Lindström, L. (2019). Can Indirect Herbicide Exposure Modify the Response of the Colorado Potato Beetle to an Organophosphate Insecticide?. Journal of Economic Entomology, 112(5), 2316-2323. https://doi.org/10.1093/jee/toz115

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Publication details

All authors or editors: Margus, Aigi; Rainio, Miia; Lindström, Leena

Journal or series: Journal of Economic Entomology

ISSN: 0022-0493

eISSN: 1938-291X

Publication year: 2019

Volume: 112

Issue number: 5

Pages range: 2316-2323

Publisher: Oxford University Press; Entomological Society of America

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1093/jee/toz115

Research data link: https://jyx.jyu.fi/handle/123456789/63500

Publication open access: Not open

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Additional information: Original data for this manuscript in JYX repository, please see: https://jyx.jyu.fi/handle/123456789/63500


Organisms live in complex multivariate environments. In agroecosystems, this complexity is often human-induced as pest individuals can be exposed to many xenobiotics simultaneously. Predicting the effects of multiple stressors can be problematic, as two or more stressors can have interactive effects. Our objective was to investigate whether indirect glyphosate-based herbicide (GBH) exposure of the host plant has interactive effects in combination with an insecticide (azinphos-methyl) on an invasive pest Colorado potato beetle (Leptinotarsa decemlineata Say). We tested the effects of GBH and insecticide on the survival, insecticide target genes expression (acetylcholinesterase genes) and oxidative status biomarkers (glutathione S-transferase [GST], glucose-6-phosphate dehydrogenase [G6PDH], glutathione reductase homolog [GR], glutathione peroxidase homolog [GPx], total glutathione [totGSH], glutathione reduced-oxidized [GSH: GSSG], catalase [CAT], superoxide dismutase [SOD], lipid hydroperoxides). We found that exposure to indirect GBH has no single or interactive effects in combination with the insecticide on larval survival. However, prior exposure to GBH inhibits Ldace1 gene expression by 0.55-fold, which is the target site for the organophosphate and carbamate insecticides. This difference disappears when individuals are exposed to both GBH and insecticide, suggesting an antagonistic effect. On the other hand, oxidative status biomarker scores (PCAs of GPx, GR, and CAT) were decreased when exposed to both stressors, indicating a synergistic effect. Overall, we found that indirect GBH exposure can have both antagonistic and synergistic effects in combination with an insecticide, which should be considered when aiming for an ecologically relevant risk assessment of multiple human-induced stressors.

Keywords: insecticides; herbicides; glyphosate; exposure; insect pests; Colorado potato beetle

Free keywords: Leptinotarsa decemlineata Say; acetylcholinesterase; azinphos-methyl; glyphosate-based herbicide (GBH); interactive effect

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Ministry reporting: Yes

Reporting Year: 2019

JUFO rating: 1

Last updated on 2021-09-06 at 13:12