A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Photoactivation of Drosophila melanogaster cryptochrome through sequential conformational transitions (2019)

Berntsson, O., Rodriguez, R., Henry, L., Panman, M. R., Hughes, A. J., Einholz, C., Weber, S., Ihalainen, J. A., Henning, R., Kosheleva, I., Schleicher, E., & Westenhoff, S. (2019). Photoactivation of Drosophila melanogaster cryptochrome through sequential conformational transitions. Science Advances, 5(7), Article eaaw1531. https://doi.org/10.1126/sciadv.aaw1531

JYU-tekijät tai -toimittajat

Ihalainen, Janne

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Berntsson, Oskar; Rodriguez, Ryan; Henry, Léocadie; Panman, Matthijs R.; Hughes, Ashley J.; Einholz, Christopher; Weber, Stefan; Ihalainen, Janne A.; Henning, Robert; Kosheleva, Irina; et al.

Lehti tai sarja: Science Advances

eISSN: 2375-2548

Julkaisuvuosi: 2019

Volyymi: 5

Lehden numero: 7

Artikkelinumero: eaaw1531

Kustantaja: American Association for the Advancement of Science

Julkaisumaa: Yhdysvallat (USA)

Julkaisun kieli: englanti

DOI: https://doi.org/10.1126/sciadv.aaw1531

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/65105

Tiivistelmä

Cryptochromes are blue-light photoreceptor proteins, which provide input to circadian clocks. The crypto-
chrome from Drosophila melanogaster (DmCry) modulates the degradation of Timeless and itself. It is unclear
how light absorption by the chromophore and the subsequent redox reactions trigger these events. Here, we
use nano- to millisecond time-resolved x-ray solution scattering to reveal the light-activated conformational
changes in DmCry and the related (6-4) photolyase. DmCry undergoes a series of structural changes, culminat-
ing in the release of the carboxyl-terminal tail (CTT). The photolyase has a simpler structural response. We find
that the CTT release in DmCry depends on pH. Mutation of a conserved histidine, important for the biochemical
activity of DmCry, does not affect transduction of the structural signal to the CTT. Instead, molecular dynamics
simulations suggest that it stabilizes the CTT in the resting-state conformation. Our structural photocycle un-
ravels the first molecular events of signal transduction in an animal cryptochrome.

YSO-asiasanat: fotobiologia; vuorokausirytmi; proteiinit; banaanikärpänen

Vapaat asiasanat: Drosophila melanogaster

Tieteenalat: