A1 Journal article (refereed)
Denitrifying microbial communities along a boreal stream with varying land-use (2019)
Aalto, S. L., Saarenheimo, J., Arvola, L., Tiirola, M., Huotari, J., & Rissanen, A. J. (2019). Denitrifying microbial communities along a boreal stream with varying land-use. Aquatic Sciences, 81(4), Article 59. https://doi.org/10.1007/s00027-019-0654-z
JYU authors or editors
Publication details
All authors or editors: Aalto, Sanni L.; Saarenheimo, Jatta; Arvola, Lauri; Tiirola, Marja; Huotari, Jussi; Rissanen, Antti J.
Journal or series: Aquatic Sciences
ISSN: 1015-1621
eISSN: 1420-9055
Publication year: 2019
Volume: 81
Issue number: 4
Article number: 59
Publisher: Birkhaeuser Science
Publication country: Switzerland
Publication language: English
DOI: https://doi.org/10.1007/s00027-019-0654-z
Publication open access: Openly available
Publication channel open access: Partially open access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/65171
Abstract
Streams have an important role in regulating nitrogen (N) transportation from terrestrial ecosystems to downstream waters. Here, we examined how catchment land-use affects potential denitrification rates and the function and composition of denitrifier communities in boreal stream sediments, using stable isotope incubations and qPCR and 454-pyrosequencing targeted on nirS, nirK and nosZ genes. Although land-use influenced the water chemistry as higher nitrite + nitrate (NO x − ) concentration at the agriculture-affected sampling point, sediment organic matter content was found to be the key factor in regulating potential denitrification rates. However, the abundance as well as the diversity and community composition of denitrifying microbes, and genetic N2O production potential (the ratio between nirS + nirK and nosZ gene abundances) were connected to both NO x − and sediment quality. Overall, our results suggest that catchment land-use-driven changes in N and carbon availability affect the denitrification rates, and possibly N2:N2O production ratio, in boreal streams, through altering denitrifier abundance and community composition.
Free keywords: catchment land-use; community composition; denitrification; nirS + nirK; nosZ; 454-pyrosequencing
Contributing organizations
Related projects
- Micro-RIP Functional analysis of uncultivated microbes using radioisotope probing
- Tiirola, Marja
- European Commission
Ministry reporting: Yes
Reporting Year: 2019
JUFO rating: 1
- Aquatic Sciences (Department of Biological and Environmental Science BIOENV) WET
- Environmental Science (Department of Biological and Environmental Science BIOENV) YMP
- Nanoscience Center (Department of Physics PHYS, JYFL) (Faculty of Mathematics and Science) (Department of Chemistry CHEM) (Department of Biological and Environmental Science BIOENV) NSC