A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
The reduction of selenium(IV) by boreal Pseudomonas sp. strain T5-6-I : Effects on selenium(IV) uptake in Brassica oleracea (2019)

Lusa, M., Help, H., Honkanen, A.-P., Knuutinen, J., Parkkonen, J., Kalasová, D., & Bomberg, M. (2019). The reduction of selenium(IV) by boreal Pseudomonas sp. strain T5-6-I : Effects on selenium(IV) uptake in Brassica oleracea. Environmental Research, 177, Article 108642. https://doi.org/10.1016/j.envres.2019.108642

JYU-tekijät tai -toimittajat

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Lusa, Merja; Help, Hanna; Honkanen, Ari-Pekka; Knuutinen, Jenna; Parkkonen, Joni; Kalasová, Dominika; Bomberg, Malin

Lehti tai sarja: Environmental Research

ISSN: 0013-9351

eISSN: 1096-0953

Julkaisuvuosi: 2019

Volyymi: 177

Artikkelinumero: 108642

Kustantaja: Elsevier

Julkaisumaa: Yhdysvallat (USA)

Julkaisun kieli: englanti

DOI: https://doi.org/10.1016/j.envres.2019.108642

Julkaisun avoin saatavuus: Ei avoin

Julkaisukanavan avoin saatavuus: 

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/65298

Julkaisu on rinnakkaistallennettu: https://tuhat.helsinki.fi/ws/portalfiles/portal/126271735/Lusa2019_postprint.pdf

Tiivistelmä

Selenium (Se) is an essential micronutrient but toxic when taken in excessive amounts. Therefore, understanding the metabolic processes related to selenium uptake and bacteria-plant interactions coupled with selenium metabolism are of high importance. We cultivated Brassica oleracea with the previously isolated heterotrophic aerobic Se(IV)-reducing Pseudomonas sp. T5-6-I strain to better understand the phenomena of bacteria-mediated Se(IV) reduction on selenium availability to the plants. B. oleracea grown on Murashige and Skoog medium (MS-salt agar) with and without of Pseudomonas sp. were amended with Se(IV)/75Se(IV), and selenium transfer into plants was studied using autoradiography and gamma spectroscopy. XANES was in addition used to study the speciation of selenium in the B. oleracea plants. In addition, the effects of Se(IV) on the protein expression in B. oleracea was studied using HPLC-SEC. TEM and confocal microscopy were used to follow the bacterial/Se-aggregate accumulation in plants and the effects of bacterial inoculation on root-hair growth. In the tests using 75Se(IV) on average 130% more selenium was translocated to the B. oleracea plants grown with Pseudomonas sp. compared to the plants grown with selenium, but without Pseudomonas sp. In addition, these bacteria notably increased root hair density. Changes in the protein expression of B. oleracea were observed on the ∼30–58 kDa regions in the Se(IV) treated samples, probably connected e.g. to the oxidative stress induced by Se(IV) or expression of proteins connected to the Se(IV) metabolism. Based on the XANES measurements, selenium appears to accumulate in B. oleracea mainly in organic C-Se-H and C-Se-C bonds with and without bacteria inoculation. We conclude that the Pseudomonas sp. T5-6-I strain seems to contribute positively to the selenium accumulation in plants, establishing the high potential of Se0-producing bacteria in the use of phytoremediation and biofortification of selenium.

YSO-asiasanat: bakteerit; seleeni; kasviekologia; ravinteet; kaalit

Vapaat asiasanat: bacteria; pseudomonas; selenium; plant uptake; bacteria-plant interactions

Liittyvät organisaatiot

OKM-raportointi: Kyllä

Raportointivuosi: 2019

JUFO-taso: 2