A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Beneficial effects of running and milk protein supplements on Sirtuins and risk factors of metabolic disorders in rats with low aerobic capacity (2019)
Lensu, S., Pekkala, S. P., Mäkinen, A., Karstunen, N., Turpeinen, A. T., Hulmi, J.J., Silvennoinen, M.M., Ma, H., Kujala, U. M., Karvinen, S., Koch, L. G., Britton, S., L., Kainulainen, H. (2019). Beneficial effects of running and milk protein supplements on Sirtuins and risk factors of metabolic disorders in rats with low aerobic capacity. Metabolism Open, 4, Article 100019. https://doi.org/10.1016/j.metop.2019.100019
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Lensu, S.; Pekkala, S. P.; Mäkinen, A.; Karstunen, N.; Turpeinen, A. T.; Hulmi, J.J.; Silvennoinen, M.M.; Ma, H.; Kujala, U. M.; Karvinen, S.; et al.
Lehti tai sarja: Metabolism Open
eISSN: 2589-9368
Julkaisuvuosi: 2019
Volyymi: 4
Artikkelinumero: 100019
Kustantaja: Elsevier
Julkaisumaa: Alankomaat
Julkaisun kieli: englanti
DOI: https://doi.org/10.1016/j.metop.2019.100019
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/66091
Tiivistelmä
Methods. Forty-seven adult LCR female rats sedentary or running voluntarily in wheels were fed normal chow and given supplements of either whey or milk protein drink (PD)-supplemented water, or water only for 21 weeks. Physiological responses were measured in vivo. Blood lipids were determined from serum. Mitochondrial markers and Sirtuins (Sirt1-7) including downstream targets were measured in plantaris muscle by western blotting.
Results. For the first 10 weeks whey-drinking rats ran about 50% less compared to other groups; still, in all runners glucose tolerance improved and triglycerides decreased. Generally, running induced a ∼six-fold increase in running capacity and a ∼8% decrease in % body fat. Together with running, protein supplements increased the relative lean mass of the total body weight by ∼11%. In comparison with sedentary controls, running and whey increased HDL (21%) and whey, with or without running, lowered LDL (-34%). Running increased mitochondrial biogenesis and Sirtuins 3 and 4. When combined with exercise, both whey and milk protein drink induced about a 4-fold increase in Sirt3, compared to runners drinking water only, and about a 2-fold increase compared to the respective sedentary group. Protein supplements, with or without running, enhanced the phosphorylation level of the acetyl-coA-carboxylase, suggesting increased fat oxidation. Both supplemented diets increased Sirt5 and Sirt7 without an additional effect from exercise. Running diminished and PD supplement increased Sirt6.
Conclusion. We demonstrate in rats new sub-chronic effects of milk proteins on metabolism that involve Sirtuins and their downstream targets in skeletal muscle. The results show that running and milk proteins act on reducing the risk factors of metabolic disorders and suggest that the underlying mechanisms may involve Sirtuins. Notably, we find milk protein supplements have some favorable effects on metabolism even without running.
YSO-asiasanat: aineenvaihdunta; lihakset; juoksu; meijerituotteet; maitovalmisteet; sirtuiinit; proteiinit; maitoproteiinit; hera
Vapaat asiasanat: low capacity running rat (LCR); metabolism; muscle; running; whey
Liittyvät organisaatiot
Hankkeet, joissa julkaisu on tehty
- Riittävä lihasmassa ja aerobinen kunto eliniän pidentäjinä lihaskadossa
- Hulmi, Juha
- Suomen Akatemia
OKM-raportointi: Kyllä
Raportointivuosi: 2019
JUFO-taso: 0