A1 Journal article (refereed)
Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection (2019)

Aho, V., Mäntylä, E., Ekman, A., Hakanen, S., Mattola, S., Chen, J.-H., Weinhardt, V., Ruokolainen, V., Sodeik, B., Larabell, C., & Vihinen-Ranta, M. (2019). Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection. Viruses, 11(10), Article 935. https://doi.org/10.3390/v11100935

JYU authors or editors

Publication details

All authors or editors: Aho, Vesa; Mäntylä, Elina; Ekman, Axel; Hakanen, Satu; Mattola, Salla; Chen, Jian-Hua; Weinhardt, Venera; Ruokolainen, Visa; Sodeik, Beate; Larabell, Carolyn; et al.

Journal or series: Viruses

eISSN: 1999-4915

Publication year: 2019

Volume: 11

Issue number: 10

Article number: 935

Publisher: MDPI

Publication country: Switzerland

Publication language: English

DOI: https://doi.org/10.3390/v11100935

Publication open access: Openly available

Publication channel open access: Open Access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/65964

Abstract

During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening around the viral replication compartments correlated with their expansion. We also observed a gradual transfer of capsids to the nuclear envelope. Later in the infection, random walk modeling indicated a gradually faster transport of capsids to the nuclear envelope that correlated with an increase in the interchromatin channels in the nuclear periphery. Our study reveals a stepwise and time-dependent mechanism of herpesvirus nuclear egress, in which progeny viral capsids approach the egress sites at the nuclear envelope via interchromatin spaces.

Keywords: herpesviruses; herpes simplex virus; infections; cell nucleus; microscopy

Free keywords: HSV-1; chromatin; nuclear egress

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Ministry reporting: Yes

Reporting Year: 2019

JUFO rating: 1