A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection (2019)


Aho, Vesa; Mäntylä, Elina; Ekman, Axel; Hakanen, Satu; Mattola, Salla; Chen, Jian-Hua; Weinhardt, Venera; Ruokolainen, Visa; Sodeik, Beate; Larabell, Carolyn; Vihinen-Ranta, Maija (2019). Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection. Viruses, 11 (10), 935. DOI: 10.3390/v11100935


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Aho, Vesa; Mäntylä, Elina; Ekman, Axel; Hakanen, Satu; Mattola, Salla; Chen, Jian-Hua; Weinhardt, Venera; Ruokolainen, Visa; Sodeik, Beate; Larabell, Carolyn; et al.

Lehti tai sarja: Viruses

eISSN: 1999-4915

Julkaisuvuosi: 2019

Volyymi: 11

Lehden numero: 10

Artikkelinumero: 935

Kustantaja: MDPI

Julkaisumaa: Sveitsi

Julkaisun kieli: englanti

DOI: https://doi.org/10.3390/v11100935

Avoin saatavuus: Open access -julkaisukanavassa ilmestynyt julkaisu

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/65964


Tiivistelmä

During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening around the viral replication compartments correlated with their expansion. We also observed a gradual transfer of capsids to the nuclear envelope. Later in the infection, random walk modeling indicated a gradually faster transport of capsids to the nuclear envelope that correlated with an increase in the interchromatin channels in the nuclear periphery. Our study reveals a stepwise and time-dependent mechanism of herpesvirus nuclear egress, in which progeny viral capsids approach the egress sites at the nuclear envelope via interchromatin spaces.


YSO-asiasanat: herpesvirukset; herpes simplex -virus; infektiot; tuma; mikroskopia

Vapaat asiasanat: HSV-1; chromatin; nuclear egress


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointi: Kyllä

Raportointivuosi: 2019

JUFO-taso: 1


Viimeisin päivitys 2020-18-08 klo 13:12