A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Cytoplasmic parvovirus capsids recruit importin beta for nuclear delivery (2020)
Mäntylä, E., Aho, V., Kann, M., & Vihinen-Ranta, M. (2020). Cytoplasmic parvovirus capsids recruit importin beta for nuclear delivery. Journal of Virology, 94(4), Article e01532-19. https://doi.org/10.1128/JVI.01532-19
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Mäntylä, Elina; Aho, Vesa; Kann, Michael; Vihinen-Ranta, Maija
Lehti tai sarja: Journal of Virology
ISSN: 0022-538X
eISSN: 1098-5514
Julkaisuvuosi: 2020
Volyymi: 94
Lehden numero: 4
Artikkelinumero: e01532-19
Kustantaja: American Society for Microbiology
Julkaisumaa: Yhdysvallat (USA)
Julkaisun kieli: englanti
DOI: https://doi.org/10.1128/JVI.01532-19
Julkaisun avoin saatavuus: Ei avoin
Julkaisukanavan avoin saatavuus:
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/66543
Tiivistelmä
Parvoviruses are an important platform for gene and cancer therapy. Their cell entry and the following steps including nuclear import are inefficient limiting their use in therapeutic applications. Two models exist on parvoviral nuclear entry: classical import of the viral capsid using nuclear transport receptors of the importin (karyopherin) family, or direct attachment of the capsid to the nuclear pore complex leading to local disintegration of the nuclear envelope. Here, by laser scanning confocal microscopy and in situ proximity ligation analysis combined with co-immunoprecipitation we showed that infection requires importin β-mediated access into the nuclear pore complex and nucleoporin 153-mediated interactions on the nuclear side. Importin β-capsid interaction continued within the nucleoplasm, which suggests that a mixed model of nuclear entry in which the classical nuclear import across the nuclear pore complex is accompanied by transient ruptures of the nuclear envelope allowing also passive entry of importin β-capsid complexes into the nucleus.
YSO-asiasanat: parvovirukset; kapsidi; solulima; tuma; karyoferiinit
Vapaat asiasanat: Parvovirus capsid; importin β; interaction; cytoplasm; nucleoplasm
Liittyvät organisaatiot
OKM-raportointi: Kyllä
Raportointivuosi: 2020
JUFO-taso: 2
