A1 Journal article (refereed)
Cytoplasmic parvovirus capsids recruit importin beta for nuclear delivery (2020)

Mäntylä, E., Aho, V., Kann, M., & Vihinen-Ranta, M. (2020). Cytoplasmic parvovirus capsids recruit importin beta for nuclear delivery. Journal of Virology, 94(4), Article e01532-19. https://doi.org/10.1128/JVI.01532-19

JYU authors or editors

Publication details

All authors or editors: Mäntylä, Elina; Aho, Vesa; Kann, Michael; Vihinen-Ranta, Maija

Journal or series: Journal of Virology

ISSN: 0022-538X

eISSN: 1098-5514

Publication year: 2020

Volume: 94

Issue number: 4

Article number: e01532-19

Publisher: American Society for Microbiology

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1128/JVI.01532-19

Publication open access: Not open

Publication channel open access:

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/66543


Parvoviruses are an important platform for gene and cancer therapy. Their cell entry and the following steps including nuclear import are inefficient limiting their use in therapeutic applications. Two models exist on parvoviral nuclear entry: classical import of the viral capsid using nuclear transport receptors of the importin (karyopherin) family, or direct attachment of the capsid to the nuclear pore complex leading to local disintegration of the nuclear envelope. Here, by laser scanning confocal microscopy and in situ proximity ligation analysis combined with co-immunoprecipitation we showed that infection requires importin β-mediated access into the nuclear pore complex and nucleoporin 153-mediated interactions on the nuclear side. Importin β-capsid interaction continued within the nucleoplasm, which suggests that a mixed model of nuclear entry in which the classical nuclear import across the nuclear pore complex is accompanied by transient ruptures of the nuclear envelope allowing also passive entry of importin β-capsid complexes into the nucleus.

Keywords: parvoviruses; capsid; cytoplasm; cell nucleus; karyopherins

Free keywords: Parvovirus capsid; importin β; interaction; cytoplasm; nucleoplasm

Contributing organizations

Ministry reporting: Yes

Reporting Year: 2020

JUFO rating: 2

Last updated on 2022-17-06 at 12:09