A1 Journal article (refereed)
Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching (2020)


Gustavsson, Emil; Isaksson, Linnéa; Persson, Cecilia; Mayzel, Maxim; Brath, Ulrika; Vrhovac, Lidija; Ihalainen, Janne A.; Karlsson, B. Göran; Orekhov, Vladislav; Westenhoff, Sebastian (2020). Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching. Biophysical Journal, 118 (2), 415-421. DOI: 10.1016/j.bpj.2019.11.025


JYU authors or editors


Publication details

All authors or editors: Gustavsson, Emil; Isaksson, Linnéa; Persson, Cecilia; Mayzel, Maxim; Brath, Ulrika; Vrhovac, Lidija; Ihalainen, Janne A.; Karlsson, B. Göran; Orekhov, Vladislav; Westenhoff, Sebastian

Journal or series: Biophysical Journal

ISSN: 0006-3495

eISSN: 1542-0086

Publication year: 2020

Volume: 118

Issue number: 2

Pages range: 415-421

Publisher: Cell Press

Publication country: United States

Publication language: English

DOI: http://doi.org/10.1016/j.bpj.2019.11.025

Open Access: Publication channel is not openly available

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/67652


Abstract

Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although crystal structures of dark and light adapted states have been determined, the molecular mechanisms underlying photoactivation remains elusive. Here we demonstrate that the conserved tongue region of the PHY domain of a 57kDa photosensory module of Deinococcus radiodurans phytochrome, changes from a structurally heterogeneous dark state to an ordered light activated state. The results were obtained in solution by utilizing a laser-triggered activation approach detected on the atomic level with high-resolution protein NMR spectroscopy. The data suggest that photosignaling of phytochromes relies on careful modulation of structural heterogeneity of the PHY tongue.


Keywords: photochemistry; proteins; molecular dynamics; NMR spectroscopy

Free keywords: phytochrome; molecular dynamics; NMR; protein structure; phototransduction


Contributing organizations


Related projects

Biologiset fotosensorit toiminnassa - Ko
Ihalainen, Janne
Academy of Finland
01/09/2016-31/08/2020


Ministry reporting: Yes

Reporting Year: 2020

Preliminary JUFO rating: 1


Last updated on 2020-18-08 at 13:16