A1 Journal article (refereed)
Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching (2020)

Gustavsson, E., Isaksson, L., Persson, C., Mayzel, M., Brath, U., Vrhovac, L., Ihalainen, J. A., Karlsson, B. G., Orekhov, V., & Westenhoff, S. (2020). Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching. Biophysical Journal, 118(2), 415-421. https://doi.org/10.1016/j.bpj.2019.11.025

JYU authors or editors

Publication details

All authors or editors: Gustavsson, Emil; Isaksson, Linnéa; Persson, Cecilia; Mayzel, Maxim; Brath, Ulrika; Vrhovac, Lidija; Ihalainen, Janne A.; Karlsson, B. Göran; Orekhov, Vladislav; Westenhoff, Sebastian

Journal or series: Biophysical Journal

ISSN: 0006-3495

eISSN: 1542-0086

Publication year: 2020

Volume: 118

Issue number: 2

Pages range: 415-421

Publisher: Cell Press

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1016/j.bpj.2019.11.025

Publication open access: Openly available

Publication channel open access: Delayed open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/67652

Abstract

Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although crystal structures of dark and light adapted states have been determined, the molecular mechanisms underlying photoactivation remains elusive. Here we demonstrate that the conserved tongue region of the PHY domain of a 57kDa photosensory module of Deinococcus radiodurans phytochrome, changes from a structurally heterogeneous dark state to an ordered light activated state. The results were obtained in solution by utilizing a laser-triggered activation approach detected on the atomic level with high-resolution protein NMR spectroscopy. The data suggest that photosignaling of phytochromes relies on careful modulation of structural heterogeneity of the PHY tongue.

Keywords: photochemistry; proteins; molecular dynamics; NMR spectroscopy

Free keywords: phytochrome; molecular dynamics; NMR; protein structure; phototransduction

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Ministry reporting: Yes

Reporting Year: 2020

JUFO rating: 1