A1 Journal article (refereed)
Assembly of Spinach Chloroplast ATP Synthase Rotor Ring Protein-Lipid Complex (2019)

Novitskaia, O., Buslaev, P., & Gushchin, I. (2019). Assembly of Spinach Chloroplast ATP Synthase Rotor Ring Protein-Lipid Complex. Frontiers in Molecular Biosciences, 6, Article 135. https://doi.org/10.3389/fmolb.2019.00135

JYU authors or editors

Publication details

All authors or editors: Novitskaia, Olga; Buslaev, Pavel; Gushchin, Ivan

Journal or series: Frontiers in Molecular Biosciences

eISSN: 2296-889X

Publication year: 2019

Volume: 6

Article number: 135

Publisher: Frontiers Media

Publication country: Switzerland

Publication language: English

DOI: https://doi.org/10.3389/fmolb.2019.00135

Publication open access: Openly available

Publication channel open access: Open Access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/67249

Abstract

Rotor ATPases are large multisubunit membrane protein complexes found in all kingdoms of life. The membrane parts of these ATPases include a ring-like assembly, so-called c-ring, consisting of several subunits c, plugged by a patch of phospholipids. In this report, we use a nature-inspired approach to model the assembly of the spinach (Spinacia oleracea) c14 ring protein-lipid complex, where partially assembled oligomers are pulled toward each other using a biasing potential. The resulting assemblies contain 23 to 26 encapsulated plug lipids, general position of which corresponds well to experimental maps. However, best fit to experimental data is achieved with 15 to 17 lipids inside the c-ring. In all of the simulations, the lipids from one leaflet (loop side of the c subunit) are ordered and static, whereas the lipids from the other leaflet are disordered and dynamic. Spontaneous permeation of water molecules toward Glu61 at the active site is also observed. The presented assembly approach is expected to be generalizable to other protein complexes with encapsulated lipid patches.

Keywords: proteins; adenosine triphosphate; lipids; cell membranes

Free keywords: membrane protein; membrane insertion; complex assembly; annular lipids; protein-lipid interactions

Contributing organizations

Ministry reporting: Yes

Reporting Year: 2019

JUFO rating: 1