A1 Journal article (refereed)
Assembly of Spinach Chloroplast ATP Synthase Rotor Ring Protein-Lipid Complex (2019)
Novitskaia, Olga; Buslaev, Pavel; Gushchin, Ivan (2019). Assembly of Spinach Chloroplast ATP Synthase Rotor Ring Protein-Lipid Complex. Frontiers in Molecular Biosciences, 6, 135. DOI: 10.3389/fmolb.2019.00135
JYU authors or editors
Publication details
All authors or editors: Novitskaia, Olga; Buslaev, Pavel; Gushchin, Ivan
Journal or series: Frontiers in Molecular Biosciences
eISSN: 2296-889X
Publication year: 2019
Volume: 6
Article number: 135
Publisher: Frontiers Media
Publication country: Switzerland
Publication language: English
DOI: https://doi.org/10.3389/fmolb.2019.00135
Open Access: Publication published in an open access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/67249
Abstract
Rotor ATPases are large multisubunit membrane protein complexes found in all kingdoms of life. The membrane parts of these ATPases include a ring-like assembly, so-called c-ring, consisting of several subunits c, plugged by a patch of phospholipids. In this report, we use a nature-inspired approach to model the assembly of the spinach (Spinacia oleracea) c14 ring protein-lipid complex, where partially assembled oligomers are pulled toward each other using a biasing potential. The resulting assemblies contain 23 to 26 encapsulated plug lipids, general position of which corresponds well to experimental maps. However, best fit to experimental data is achieved with 15 to 17 lipids inside the c-ring. In all of the simulations, the lipids from one leaflet (loop side of the c subunit) are ordered and static, whereas the lipids from the other leaflet are disordered and dynamic. Spontaneous permeation of water molecules toward Glu61 at the active site is also observed. The presented assembly approach is expected to be generalizable to other protein complexes with encapsulated lipid patches.
Keywords: proteins; adenosine triphosphate; lipids; cell membranes
Free keywords: membrane protein; membrane insertion; complex assembly; annular lipids; protein-lipid interactions
Contributing organizations
Ministry reporting: Yes
Reporting Year: 2019
JUFO rating: 1
