A1 Journal article (refereed)
Osmotrophic glucose and leucine assimilation and its impact on EPA and DHA content in algae (2020)


Peltomaa, E. T., & Taipale, S. (2020). Osmotrophic glucose and leucine assimilation and its impact on EPA and DHA content in algae. PeerJ, 8, Article e8363. https://doi.org/10.7717/peerj.8363


JYU authors or editors


Publication details

All authors or editors: Peltomaa, Elina T.; Taipale, Sami

Journal or series: PeerJ

eISSN: 2167-8359

Publication year: 2020

Volume: 8

Article number: e8363

Publisher: PeerJ, Ltd.

Publication country: United Kingdom

Publication language: English

DOI: https://doi.org/10.7717/peerj.8363

Research data link: http://dx.doi.org/10.7717/ peerj.8363#supplemental-information

Publication open access: Openly available

Publication channel open access: Open Access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/67267


Abstract

The uptake of dissolved organic compounds, that is, osmotrophy, has been shown to be an efficient nutritional strategy for algae. However, this mode of nutrition may affect the biochemical composition, for example, the fatty acid (FA) contents, of algal cells. This study focused on the osmotrophic assimilation of glucose and leucine by selected seven algal strains belonging to chlorophytes, chrysophytes, cryptophytes, dinoflagellates and euglenoids. Our laboratory experiments with stable isotope labeling showed that osmotrophy occurred in four of the selected seven strains. However, only three of these produced long chain omega-3 FAs eicosapentaenoic acid (EPA; 20:5ω3) and docosahexaenoic acid (DHA; 22:6ω3). High glucose content (5 mg L−1) affected negatively on the total FAs of Mallomonas kalinae and the total omega-3 FAs of Cryptomonas sp. Further, glucose assimilation explained 35% (negative effect) and leucine assimilation 48% (positive effect) of the variation of EPA, DHA and the FAs related to their synthesis in Cryptomonas sp. Moderate glucose concentration (2 mg L−1) was found to enhance the growth of Cryptomonas ozolinii, whereas low leucine (20 µg L−1) enhanced the growth of M. kalinae. However, no systematic effect of osmotrophy on growth rates was detected. Our study shows that osmotrophic assimilation of algae is species and compound specific, and that the effects of the assimilated compounds on algal metabolism also varies depending on the species.


Keywords: omega fatty acids; isotopes; algae

Free keywords: mixotrophy; omega-3 fatty acids; stable isotope labeling; cryptophytes


Contributing organizations


Ministry reporting: Yes

Reporting Year: 2020

JUFO rating: 1


Last updated on 2022-20-09 at 15:55