A1 Journal article (refereed)
Dispersion from Cα or NH : 4D experiments for backbone resonance assignment of intrinsically disordered proteins (2020)


Tossavainen, H., Salovaara, S., Hellman, M., Ihalin, R., & Permi, P. (2020). Dispersion from Cα or NH : 4D experiments for backbone resonance assignment of intrinsically disordered proteins. Journal of Biomolecular NMR, 74(2-3), 147-159. https://doi.org/10.1007/s10858-020-00299-w


JYU authors or editors


Publication details

All authors or editorsTossavainen, Helena; Salovaara, Santeri; Hellman, Maarit; Ihalin, Riikka; Permi, Perttu

Journal or seriesJournal of Biomolecular NMR

ISSN0925-2738

eISSN1573-5001

Publication year2020

Volume74

Issue number2-3

Pages range147-159

PublisherSpringer

Publication countryNetherlands

Publication languageEnglish

DOIhttps://doi.org/10.1007/s10858-020-00299-w

Publication open accessOpenly available

Publication channel open accessPartially open access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/68258


Abstract

Resonance assignment of intrinsically disordered proteins is remarkably challenging due to scant chemical shift dispersion arising from conformational heterogeneity. The challenge is even greater if repeating segments are present in the amino acid sequence. To forward unambiguous resonance assignment of intrinsically disordered proteins, we present iHACANCO, HACACON and (HACA)CONCAHA, three Hα-detected 4D experiments with Cα as an additional dimension. In addition, we present (HACA)CON(CA)NH and (HACA)N(CA)CONH, new 4D Hα-start, HN-detect experiments which have two NH dimensions to enhance peak dispersion in a sequential walk through C', NH and HN, and provide more accurate NH/HN chemical shifts than those that can be obtained from a crowded 1H, 15N-HSQC spectrum. Application of these 4D experiments is demonstrated using BilRI (165 aa), an outer-membrane intrinsically disordered protein from the opportunistic oral pathogen Aggregatibacter actinomycetemcomitans. BilRI amino acid sequence encompasses three very similar repeats with a 13-residue identical stretch in two of them.


KeywordsproteinsbacteriaresonanceNMR spectroscopy

Free keywordsAggregatibacter actinomycetemcomitans; BilRI; IDP; intrinsically disordered protein; resonance assignment


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Ministry reportingYes

Reporting Year2020

JUFO rating1


Last updated on 2024-22-04 at 12:05