A1 Journal article (refereed)
Further insights in the Tardigrada microbiome : phylogenetic position and prevalence of infection of four new Alphaproteobacteria putative endosymbionts (2020)


Guidetti, Roberto; Vecchi, Matteo; Ferrari, Agnese; Newton, Irene L G; Cesari, Michele; Rebecchi, Lorena (2020). Further insights in the Tardigrada microbiome : phylogenetic position and prevalence of infection of four new Alphaproteobacteria putative endosymbionts. Zoological Journal of the Linnean Society, 188 (3), 925-937. DOI: 10.1093/zoolinnean/zlz128


JYU authors or editors


Publication details

All authors or editors: Guidetti, Roberto; Vecchi, Matteo; Ferrari, Agnese; Newton, Irene L G; Cesari, Michele; Rebecchi, Lorena

Journal or series: Zoological Journal of the Linnean Society

ISSN: 0024-4082

eISSN: 1096-3642

Publication year: 2020

Volume: 188

Issue number: 3

Pages range: 925-937

Publisher: Wiley-Blackwell

Publication country: United Kingdom

Publication language: English

DOI: https://doi.org/10.1093/zoolinnean/zlz128

Open Access: Publication channel is not openly available

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/68862


Abstract

Data from a previous study showed that microbiomes of six tardigrade species are species-specific and distinct from associated environmental microbes. We here performed a more in-depth analyses of those data, to identify and characterize new potential symbionts. The most abundant bacterial operational taxonomic units (OTUs) found in tardigrades are classified, and their prevalence in other environments is assessed using public databases. A subset of OTUs was selected for molecular phylogenetic analyses based on their affiliation with host-associated bacterial families in tardigrades. Almost 22.6% of the most abundant OTUs found do not match any sequence at 99% identity in the IMNGS database. These novel OTUs include four putative tardigrade endosymbionts from Alphaproteobacteria (Anaplasmataceae and Candidatus Tenuibacteraceae), which are characterized by 16S rRNA gene analysis and investigated for their infection rates in: Echiniscus trisetosus, Richtersisus coronifer and Macrobiotus macrocalix. These putative endosymbionts have an infection prevalence between 9.1% and 40.0%, and are, therefore, likely secondary symbionts, not essential for tardigrade survival and reproduction. Using fluorescence in situ hybridization (FISH), we detected bacteria on the cuticle and within the ovary of E. trisetosus, suggesting possible vertical transmission. This study highlights the great contribution in biodiversity discovery that neglected phyla can provide in microbiome and symbiosis studies.


Keywords: bacteria; biomes

Free keywords: Rickettsiales; Holosporales; Anaplasmataceae, Ca. Tenuibacteraceae; Echiniscus trisetosus; Macrobiotus macrocalix; Richtersius coronifer; FISH; operational taxonomic units


Contributing organizations


Ministry reporting: Yes

Reporting Year: 2020

Preliminary JUFO rating: 1


Last updated on 2020-18-08 at 13:20