A1 Journal article (refereed)
Sprint and Strength Training Modulates Autophagy and Proteostasis in Aging Sprinters (2020)

Hentilä, J., Hulmi, J. J., Laakkonen, E. K., Ahtiainen, J. P., Suominen, H., & Korhonen, M. T. (2020). Sprint and Strength Training Modulates Autophagy and Proteostasis in Aging Sprinters. Medicine and Science in Sports and Exercise, 52(9), 1948-1959. https://doi.org/10.1249/MSS.0000000000002340

JYU authors or editors

Publication details

All authors or editors: Hentilä, Jaakko; Hulmi, Juha J.; Laakkonen, Eija K.; Ahtiainen, Juha P.; Suominen, Harri; Korhonen, Marko T.

Journal or series: Medicine and Science in Sports and Exercise

ISSN: 0195-9131

eISSN: 1530-0315

Publication year: 2020

Volume: 52

Issue number: 9

Pages range: 1948-1959

Publisher: Lippincott Williams & Wilkins

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1249/MSS.0000000000002340

Publication open access: Not open

Publication channel open access:

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/68298


Exercise and aging may modulate muscle protein homeostasis and autophagy, but few studies examine highly-trained middle-aged or older individuals. This study elucidated the effects of a new long-term training stimulus on markers of muscle autophagy and unfolded protein response (UPR) and on sprint running performance in masters sprinters.
Thirty-two male competitive sprinters (aged 40–76 years) were randomly divided into experimental (EX) and control (CTRL) groups. The EX training program was a combination of heavy and explosive strength and sprint exercises aimed at improving sprint performance. Fifteen and thirteen participants completed the 20-week intervention period in EX and CTRL, respectively. The latter were told to continue their routine exercises. Key protein markers were analyzed by western blotting from vastus lateralis (VL) muscle biopsies. Muscle thickness of VL was analyzed by ultrasonography and sprint performance by a 60-meter running test.
EX induced improvement in 60-meter sprint performance when compared to controls (time x group, P = 0.003) without changes in VL muscle thickness. Content of lipidated microtubule-associated protein 1A/1B-light chain 3 (LC3-II) increased in EX (P = 0.022) suggesting increased autophagosome content. Additionally, an autophagosome clearance marker sequestosome 1 (p62) decreased in EX (P = 0.006). Markers of UPR selectively modulated with decreases (e.g. ATF4, P = 0.003) and increases (e.g. EIF2α, P = 0.019) observed in EX.
These findings suggest that a new intensive training stimulus that combines strength training with sprint training may increase muscle autophagosome content in a basal state without any evidence of impaired autophagosome clearance in masters sprinters. Simultaneously, the combined training may have a selective effect on the content of UPR signaling components.

Keywords: ageing; athletes; muscles; cell physiology; proteins

Free keywords: masters athlete; skeletal muscle; unfolded protein response

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Related projects

Ministry reporting: Yes

Reporting Year: 2020

JUFO rating: 3

Last updated on 2022-14-09 at 12:07