A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Nitrate removal microbiology in woodchip bioreactors : a case-study with full-scale bioreactors treating aquaculture effluents (2020)

Aalto, S. L., Suurnäkki, S., von Ahnen, M., Siljanen, H. M. P., Pedersen, P. B., & Tiirola, M. (2020). Nitrate removal microbiology in woodchip bioreactors : a case-study with full-scale bioreactors treating aquaculture effluents. Science of the Total Environment, 723, Article 138093. https://doi.org/10.1016/j.scitotenv.2020.138093

JYU-tekijät tai -toimittajat

Aalto, Sanni
Suurnäkki, Suvi
Tiirola, Marja

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Aalto, Sanni L.; Suurnäkki, Suvi; von Ahnen, Mathis; Siljanen, Henri M. P.; Pedersen, Per Bovbjerg; Tiirola, Marja

Lehti tai sarja: Science of the Total Environment

ISSN: 0048-9697

eISSN: 1879-1026

Julkaisuvuosi: 2020

Volyymi: 723

Artikkelinumero: 138093

Kustantaja: Elsevier

Julkaisumaa: Alankomaat

Julkaisun kieli: englanti

DOI: https://doi.org/10.1016/j.scitotenv.2020.138093

Julkaisun avoin saatavuus: Ei avoin

Julkaisukanavan avoin saatavuus: 

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/68374

Tiivistelmä

Woodchip bioreactors are viable low-cost nitrate (NO3−) removal applications for treating agricultural and aquaculture discharges. The active microbial biofilms growing on woodchips are conducting nitrogen (N) removal, reducing NO3− while oxidizing the carbon (C) from woodchips. However, bioreactor age, and changes in the operating conditions or in the microbial community might affect the NO3− removal as well as potentially promote nitrous oxide (N2O) production through either incomplete denitrification or dissimilatory NO3− reduction to ammonium (DNRA). Here, we combined stable isotope approach, amplicon sequencing, and captured metagenomics for studying the potential NO3− removal rates, and the abundance and community composition of microbes involved in N transformation processes in the three different full-scale woodchip bioreactors treating recirculating aquaculture system (RAS) effluents. We confirmed denitrification producing di‑nitrogen gas (N2) to be the primary NO3− removal pathway, but found that 6% of NO3− could be released as N2O under high NO3− concentrations and low amounts of bioavailable C, whereas DNRA rates tend to increase with the C amount. The abundance of denitrifiers was equally high between the studied bioreactors, yet the potential NO3− removal rates were linked to the denitrifying community diversity. The same core proteobacterial groups were driving the denitrification, while Bacteroidetes dominated the DNRA carrying microbes in all the three bioreactors studied. Altogether, our results suggest that woodchip bioreactors have a high genetic potential for NO3− removal through a highly abundant and diverse denitrifying community, but that the rates and dynamics between the NO3− removal pathways depend on the other factors (e.g., bioreactor design, operating conditions, and the amount of bioavailable C in relation to the incoming NO3− concentrations).

YSO-asiasanat: denitrifikaatio; dityppioksidi; typpi; bioreaktorit; vesiviljely (kalatalous)

Vapaat asiasanat: denitrification; DNRA; nitrogen removal; nitrous oxide; recirculation aquaculture systems

Liittyvät organisaatiot

Hankkeet, joissa julkaisu on tehty

OKM-raportointi: Kyllä

Raportointivuosi: 2020

JUFO-taso: 2