A1 Journal article (refereed)
Early entry events in Echovirus 30 infection (2020)


Vandesande, Helena; Laajala, Mira; Kantoluoto, Tino; Ruokolainen, Visa; Lindberg, A. Michael; Marjomäki, Varpu (2020). Early entry events in Echovirus 30 infection. Journal of Virology, 94 (13), e00592-20. DOI: 10.1128/JVI.00592-20


JYU authors or editors


Publication details

All authors or editors: Vandesande, Helena; Laajala, Mira; Kantoluoto, Tino; Ruokolainen, Visa; Lindberg, A. Michael; Marjomäki, Varpu

Journal or series: Journal of Virology

ISSN: 0022-538X

eISSN: 1098-5514

Publication year: 2020

Volume: 94

Issue number: 13

Article number: e00592-20

Publisher: American Society for Microbiology

Publication country: United States

Publication language: English

DOI: http://doi.org/10.1128/JVI.00592-20

Open Access: Publication channel is not openly available

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/68681


Abstract

Echovirus 30 (E30), a member of the enterovirus B species, is a major cause of viral meningitis, targeting children and adults alike. While it is a frequently isolated enterovirus and the cause of several outbreaks all over the world, suprisingly little is known regarding its entry and replication strategy within cells. In this study, we used E30 Bastianni (E30B) generated from an infectious cDNA clone in order to study early entry events during infection in human RD cells. E30B required the newly discovered Fc echovirus receptor (FcRn) for succesful infection, but not the Coxsackievirus and Adenovirus Receptor (CAR) or Decay-Accelerating Factor (DAF), although an interaction with DAF was observed. Double-stranded RNA replication intermediate was generated between 2 and 3 h post-infection (p.i.). and viral capsid production was initiated between 4 and 5 h p.i. The drugs affecting Rac1 (NSC 23766) and cholesterol (Filipin III) compromised infection, whereas bafilomycin A1, dyngo, U-73122, wortmannin and nocodazole did not, suggesting the virus follows an enterovirus-triggered macropinocytic pathway rather than the clathrin pathway. Colocalization with early endosomes and increased infection due to constitutively active Rab5 expression suggests some overlap and entry to classical early endosomes. Taken together, these results suggest that E30B induces an enterovirus entry pathway, leading to uncoating in early endosomes.


Keywords: enteroviruses; ECHO viruses; infections; meningitis

Free keywords: enterovirus; echovirus; aseptic meningitis; early entry


Contributing organizations


Ministry reporting: Yes

Reporting Year: 2020

Preliminary JUFO rating: 2


Last updated on 2020-18-08 at 13:32