A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Early entry events in Echovirus 30 infection (2020)


Vandesande, Helena; Laajala, Mira; Kantoluoto, Tino; Ruokolainen, Visa; Lindberg, A. Michael; Marjomäki, Varpu (2020). Early entry events in Echovirus 30 infection. Journal of Virology, 94 (13), e00592-20. DOI: 10.1128/JVI.00592-20


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Vandesande, Helena; Laajala, Mira; Kantoluoto, Tino; Ruokolainen, Visa; Lindberg, A. Michael; Marjomäki, Varpu

Lehti tai sarja: Journal of Virology

ISSN: 0022-538X

eISSN: 1098-5514

Julkaisuvuosi: 2020

Volyymi: 94

Lehden numero: 13

Artikkelinumero: e00592-20

Kustantaja: American Society for Microbiology

Julkaisumaa: Yhdysvallat (USA)

Julkaisun kieli: englanti

DOI: https://doi.org/10.1128/JVI.00592-20

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Julkaisun avoin saatavuus:

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/68681


Tiivistelmä

Echovirus 30 (E30), a member of the enterovirus B species, is a major cause of viral meningitis, targeting children and adults alike. While it is a frequently isolated enterovirus and the cause of several outbreaks all over the world, suprisingly little is known regarding its entry and replication strategy within cells. In this study, we used E30 Bastianni (E30B) generated from an infectious cDNA clone in order to study early entry events during infection in human RD cells. E30B required the newly discovered Fc echovirus receptor (FcRn) for succesful infection, but not the Coxsackievirus and Adenovirus Receptor (CAR) or Decay-Accelerating Factor (DAF), although an interaction with DAF was observed. Double-stranded RNA replication intermediate was generated between 2 and 3 h post-infection (p.i.). and viral capsid production was initiated between 4 and 5 h p.i. The drugs affecting Rac1 (NSC 23766) and cholesterol (Filipin III) compromised infection, whereas bafilomycin A1, dyngo, U-73122, wortmannin and nocodazole did not, suggesting the virus follows an enterovirus-triggered macropinocytic pathway rather than the clathrin pathway. Colocalization with early endosomes and increased infection due to constitutively active Rab5 expression suggests some overlap and entry to classical early endosomes. Taken together, these results suggest that E30B induces an enterovirus entry pathway, leading to uncoating in early endosomes.


YSO-asiasanat: enterovirukset; ECHO-virukset; infektiot; aivokalvotulehdus

Vapaat asiasanat: enterovirus; echovirus; aseptic meningitis; early entry


Liittyvät organisaatiot


OKM-raportointi: Kyllä

Raportointivuosi: 2020

Alustava JUFO-taso: 2


Viimeisin päivitys 2020-18-08 klo 13:32