A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery (2020)

Saari, H., Turunen, T., Lõhmus, A., Turunen, M., Jalasvuori, M., Butcher, S. J., Ylä-Herttuala, S., Viitala, T., Cerullo, V., Siljander, P. R. M., & Yliperttula, M. (2020). Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery. Journal of Extracellular Vesicles, 9(1), Article 1747206. https://doi.org/10.1080/20013078.2020.1747206

JYU-tekijät tai -toimittajat

Jalasvuori, Matti

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Saari, Heikki; Turunen, Tiia; Lõhmus, Andres; Turunen, Mikko; Jalasvuori, Matti; Butcher, Sarah J.; Ylä-Herttuala, Seppo; Viitala, Tapani; Cerullo, Vincenzo; Siljander, Pia R. M.; et al.

Lehti tai sarja: Journal of Extracellular Vesicles

eISSN: 2001-3078

Julkaisuvuosi: 2020

Volyymi: 9

Lehden numero: 1

Artikkelinumero: 1747206

Kustantaja: Taylor & Francis

Julkaisumaa: Britannia

Julkaisun kieli: englanti

DOI: https://doi.org/10.1080/20013078.2020.1747206

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/68675

Tiivistelmä

Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy.

YSO-asiasanat: adenovirukset; onkolyyttiset virukset; syöpähoidot; onkolyyttinen virushoito; solukalvot

Vapaat asiasanat: extracellular vesicles; adenovirus; cancer therapy; DNA delivery

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