A1 Journal article (refereed)
Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery (2020)

Saari, H., Turunen, T., Lõhmus, A., Turunen, M., Jalasvuori, M., Butcher, S. J., Ylä-Herttuala, S., Viitala, T., Cerullo, V., Siljander, P. R. M., & Yliperttula, M. (2020). Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery. Journal of Extracellular Vesicles, 9(1), Article 1747206. https://doi.org/10.1080/20013078.2020.1747206

JYU authors or editors

Jalasvuori, Matti

Publication details

All authors or editors: Saari, Heikki; Turunen, Tiia; Lõhmus, Andres; Turunen, Mikko; Jalasvuori, Matti; Butcher, Sarah J.; Ylä-Herttuala, Seppo; Viitala, Tapani; Cerullo, Vincenzo; Siljander, Pia R. M.; et al.

Journal or series: Journal of Extracellular Vesicles

eISSN: 2001-3078

Publication year: 2020

Volume: 9

Issue number: 1

Article number: 1747206

Publisher: Taylor & Francis

Publication country: United Kingdom

Publication language: English

DOI: https://doi.org/10.1080/20013078.2020.1747206

Publication open access: Openly available

Publication channel open access: Open Access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/68675

Abstract

Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy.

Keywords: adenoviruses; oncolytic viruses; cancer treatments; oncolytic virotherapy; cell membranes

Free keywords: extracellular vesicles; adenovirus; cancer therapy; DNA delivery

Fields of science: