A1 Journal article (refereed)
Differentiation of Murine C2C12 Myoblasts Strongly Reduces the Effects of Myostatin on Intracellular Signaling (2020)


Lautaoja, J. H., Pekkala, S., Pasternack, A., Laitinen, M., Ritvos, O., & Hulmi, J. J. (2020). Differentiation of Murine C2C12 Myoblasts Strongly Reduces the Effects of Myostatin on Intracellular Signaling. Biomolecules, 10(5), Article 695. https://doi.org/10.3390/biom10050695


JYU authors or editors


Publication details

All authors or editorsLautaoja, Juulia H.; Pekkala, Satu; Pasternack, Arja; Laitinen, Mika; Ritvos, Olli; Hulmi, Juha J.

Journal or seriesBiomolecules

eISSN2218-273X

Publication year2020

Volume10

Issue number5

Article number695

PublisherMDPI

Publication countrySwitzerland

Publication languageEnglish

DOIhttps://doi.org/10.3390/biom10050695

Publication open accessOpenly available

Publication channel open accessOpen Access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/68893


Abstract

Alongside in vivo models, a simpler and more mechanistic approach is required to study the effects of myostatin on skeletal muscle because myostatin is an important negative regulator of muscle size. In this study, myostatin was administered to murine (C2C12) and human (CHQ) myoblasts and myotubes. Canonical and noncanonical signaling downstream to myostatin, related ligands, and their receptor were analyzed. The effects of tumorkines were analyzed after coculture of C2C12 and colon cancer-C26 cells. The effects of myostatin on canonical and noncanonical signaling were strongly reduced in C2C12 cells after differentiation. This may be explained by increased follistatin, an endogenous blocker of myostatin and altered expression of activin receptor ligands. In contrast, CHQ cells were equally responsive to myostatin, and follistatin remained unaltered. Both myostatin administration and the coculture stimulated pathways associated with inflammation, especially in C2C12 cells. In conclusion, the effects of myostatin on intracellular signaling may be cell line- or organism-specific, and C2C12 myotubes seem to be a nonoptimal in vitro model for investigating the effects of myostatin on canonical and noncanonical signaling in skeletal muscle. This may be due to altered expression of activin receptor ligands and their regulators during muscle cell differentiation.


Keywordsmusclesmuscle cellscell signalingproteins

Free keywordscoculture; follistatin; inflammation; MAPK; myotube; skeletal muscle; Smad; tumorkine


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Ministry reportingYes

Reporting Year2020

JUFO rating1


Last updated on 2024-03-04 at 21:27