A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Beta-Lactam Sensitive Bacteria Can Acquire ESBL-Resistance via Conjugation after Long-Term Exposure to Lethal Antibiotic Concentration (2020)


Ruotsalainen, Pilvi; Given, Cindy; Penttinen, Reetta; Jalasvuori, Matti (2020). Beta-Lactam Sensitive Bacteria Can Acquire ESBL-Resistance via Conjugation after Long-Term Exposure to Lethal Antibiotic Concentration. Antibiotics, 9 (6), 296. DOI: 10.3390/antibiotics9060296


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Ruotsalainen, Pilvi; Given, Cindy; Penttinen, Reetta; Jalasvuori, Matti

Lehti tai sarja: Antibiotics

eISSN: 2079-6382

Julkaisuvuosi: 2020

Volyymi: 9

Lehden numero: 6

Artikkelinumero: 296

Kustantaja: MDPI

Julkaisumaa: Sveitsi

Julkaisun kieli: englanti

DOI: https://doi.org/10.3390/antibiotics9060296

Avoin saatavuus: Open access -julkaisukanavassa ilmestynyt julkaisu

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/69784


Tiivistelmä

Beta-lactams are commonly used antibiotics that prevent cell-wall biosynthesis. Beta-lactam sensitive bacteria can acquire conjugative resistance elements and hence become resistant even after being exposed to lethal (above minimum inhibitory) antibiotic concentrations. Here we show that neither the length of antibiotic exposure (1 to 16 h) nor the beta-lactam type (penam or cephem) have a major impact on the rescue of sensitive bacteria. We demonstrate that an evolutionary rescue can occur between different clinically relevant bacterial species (Klebsiella pneumoniae and Escherichia coli) by plasmids that are commonly associated with extended-spectrum beta-lactamase (ESBL) positive hospital isolates. As such, it is possible that this resistance dynamic may play a role in failing antibiotic therapies in those cases where resistant bacteria may readily migrate into the proximity of sensitive pathogens. Furthermore, we engineered a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) -plasmid to encode a guiding CRISPR-RNA against the migrating ESBL-plasmid. By introducing this plasmid into the sensitive bacterium, the frequency of the evolutionarily rescued bacteria decreased by several orders of magnitude. As such, engineering pathogens during antibiotic treatment may provide ways to prevent ESBL-plasmid dispersal and hence resistance evolution.


YSO-asiasanat: bakteerit; Klebsiella-bakteerit; kolibakteerit; antibioottiresistenssi; antibiootit; horisontaalinen geeninsiirto; plasmidit

Vapaat asiasanat: antibiotic resistance; extended-spectrum beta-lactamase; evolutionary rescue; conjugative plasmid


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointi: Kyllä

Raportointivuosi: 2020

Alustava JUFO-taso: 1


Viimeisin päivitys 2021-02-02 klo 09:49