A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor (2020)

Duanghathaipornsuk, S., Shen, B., Cameron, B. D., Ijäs, H., Linko, V., Kostiainen, M. A., & Kim, D.-S. (2020). Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor. Materials Letters, 275, Article 128141. https://doi.org/10.1016/j.matlet.2020.128141

JYU-tekijät tai -toimittajat

Ijäs, Heini

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Duanghathaipornsuk, Surachet; Shen, Boxuan; Cameron, Brent D.; Ijäs, Heini; Linko, Veikko; Kostiainen, Mauri A.; Kim, Dong-Shik

Lehti tai sarja: Materials Letters

ISSN: 0167-577X

eISSN: 1873-4979

Julkaisuvuosi: 2020

Volyymi: 275

Artikkelinumero: 128141

Kustantaja: Elsevier

Julkaisumaa: Alankomaat

Julkaisun kieli: englanti

DOI: https://doi.org/10.1016/j.matlet.2020.128141

Julkaisun avoin saatavuus: Ei avoin

Julkaisukanavan avoin saatavuus:

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/70228

Tiivistelmä

DNA origami-based cages functionalized with aptamer motifs, were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.

YSO-asiasanat: nanorakenteet; DNA; plasmonit; biosensorit; hemoglobiini

Vapaat asiasanat: DNA origami; glycated hemoglobin; aptamer; surface plasmon resonance sensor; dissociation constant

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