A1 Journal article (refereed)
Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor (2020)


Duanghathaipornsuk, Surachet; Shen, Boxuan; Cameron, Brent D.; Ijäs, Heini; Linko, Veikko; Kostiainen, Mauri A.; Kim, Dong-Shik (2020). Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor. Materials Letters, 275, 128141. DOI: 10.1016/j.matlet.2020.128141


JYU authors or editors


Publication details

All authors or editors: Duanghathaipornsuk, Surachet; Shen, Boxuan; Cameron, Brent D.; Ijäs, Heini; Linko, Veikko; Kostiainen, Mauri A.; Kim, Dong-Shik

Journal or series: Materials Letters

ISSN: 0167-577X

eISSN: 1873-4979

Publication year: 2020

Volume: 275

Article number: 128141

Publisher: Elsevier

Publication country: Netherlands

Publication language: English

DOI: http://doi.org/10.1016/j.matlet.2020.128141

Open Access: Publication channel is not openly available

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/70228


Abstract

DNA origami-based cages functionalized with aptamer motifs, were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.


Keywords: nanostructures; DNA; plasmons; biosensors; hemoglobin

Free keywords: DNA origami; glycated hemoglobin; aptamer; surface plasmon resonance sensor; dissociation constant


Contributing organizations


Ministry reporting: Yes

Reporting Year: 2020


Last updated on 2020-18-08 at 13:22