A1 Journal article (refereed)
Adaptation of gene loci to heterochromatin in the course of Drosophila evolution is associated with insulator proteins (2020)


Funikov, Sergei Yu.; Rezvykh, Alexander P.; Kulikova, Dina A.; Zelentsova, Elena S.; Protsenko, Lyudmila A.; Chuvakova, Lyubov N.; Tyukmaeva, Venera I.; Arkhipova, Irina R.; Evgen’ev, Michael B. (2020). Adaptation of gene loci to heterochromatin in the course of Drosophila evolution is associated with insulator proteins. Scientific Reports, 10, 11893. DOI: 10.1038/s41598-020-68879-2


JYU authors or editors


Publication details

All authors or editors: Funikov, Sergei Yu.; Rezvykh, Alexander P.; Kulikova, Dina A.; Zelentsova, Elena S.; Protsenko, Lyudmila A.; Chuvakova, Lyubov N.; Tyukmaeva, Venera I.; Arkhipova, Irina R.; Evgen’ev, Michael B.

Journal or series: Scientific Reports

eISSN: 2045-2322

Publication year: 2020

Volume: 10

Article number: 11893

Publisher: Nature Publishing Group

Publication country: United Kingdom

Publication language: English

DOI: http://doi.org/10.1038/s41598-020-68879-2

Open Access: Publication published in an open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/71234


Abstract

Pericentromeric heterochromatin is generally composed of repetitive DNA forming a transcriptionally repressive environment. Dozens of genes were embedded into pericentromeric heterochromatin during evolution of Drosophilidae lineage while retaining activity. However, factors that contribute to insusceptibility of gene loci to transcriptional silencing remain unknown. Here, we find that the promoter region of genes that can be embedded in both euchromatin and heterochromatin exhibits a conserved structure throughout the Drosophila phylogeny and carries motifs for binding of certain chromatin remodeling factors, including insulator proteins. Using ChIP-seq data, we demonstrate that evolutionary gene relocation between euchromatin and pericentric heterochromatin occurred with preservation of sites of insulation of BEAF-32 in evolutionarily distant species, i.e. D. melanogaster and D. virilis. Moreover, promoters of virtually all protein-coding genes located in heterochromatin in D. melanogaster are enriched with insulator proteins BEAF-32, GAF and dCTCF. Applying RNA-seq of a BEAF-32 mutant, we show that the impairment of BEAF-32 function has a complex effect on gene expression in D. melanogaster, affecting even those genes that lack BEAF-32 association in their promoters. We propose that conserved intrinsic properties of genes, such as sites of insulation near the promoter regions, may contribute to adaptation of genes to the heterochromatic environment and, hence, facilitate the evolutionary relocation of genes loci between euchromatin and heterochromatin.


Keywords: genes; DNA


Contributing organizations


Ministry reporting: Yes


Last updated on 2020-27-07 at 11:35