A1 Journal article (refereed)
Adaptation of gene loci to heterochromatin in the course of Drosophila evolution is associated with insulator proteins (2020)
Funikov, S. Y., Rezvykh, A. P., Kulikova, D. A., Zelentsova, E. S., Protsenko, L. A., Chuvakova, L. N., Tyukmaeva, V. I., Arkhipova, I. R., & Evgen’ev, M. B. (2020). Adaptation of gene loci to heterochromatin in the course of Drosophila evolution is associated with insulator proteins. Scientific Reports, 10, Article 11893. https://doi.org/10.1038/s41598-020-68879-2
JYU authors or editors
Publication details
All authors or editors: Funikov, Sergei Yu.; Rezvykh, Alexander P.; Kulikova, Dina A.; Zelentsova, Elena S.; Protsenko, Lyudmila A.; Chuvakova, Lyubov N.; Tyukmaeva, Venera I.; Arkhipova, Irina R.; Evgen’ev, Michael B.
Journal or series: Scientific Reports
eISSN: 2045-2322
Publication year: 2020
Publication date: 17/07/2020
Volume: 10
Article number: 11893
Publisher: Nature Publishing Group
Publication country: United Kingdom
Publication language: English
DOI: https://doi.org/10.1038/s41598-020-68879-2
Publication open access: Openly available
Publication channel open access: Open Access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/71234
Abstract
Pericentromeric heterochromatin is generally composed of repetitive DNA forming a transcriptionally repressive environment. Dozens of genes were embedded into pericentromeric heterochromatin during evolution of Drosophilidae lineage while retaining activity. However, factors that contribute to insusceptibility of gene loci to transcriptional silencing remain unknown. Here, we find that the promoter region of genes that can be embedded in both euchromatin and heterochromatin exhibits a conserved structure throughout the Drosophila phylogeny and carries motifs for binding of certain chromatin remodeling factors, including insulator proteins. Using ChIP-seq data, we demonstrate that evolutionary gene relocation between euchromatin and pericentric heterochromatin occurred with preservation of sites of insulation of BEAF-32 in evolutionarily distant species, i.e. D. melanogaster and D. virilis. Moreover, promoters of virtually all protein-coding genes located in heterochromatin in D. melanogaster are enriched with insulator proteins BEAF-32, GAF and dCTCF. Applying RNA-seq of a BEAF-32 mutant, we show that the impairment of BEAF-32 function has a complex effect on gene expression in D. melanogaster, affecting even those genes that lack BEAF-32 association in their promoters. We propose that conserved intrinsic properties of genes, such as sites of insulation near the promoter regions, may contribute to adaptation of genes to the heterochromatic environment and, hence, facilitate the evolutionary relocation of genes loci between euchromatin and heterochromatin.
Contributing organizations
Ministry reporting: Yes
VIRTA submission year: 2020
JUFO rating: 1
