A1 Journal article (refereed)
Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics (2020)
Rigaud, C., Eriksson, A., Krasnov, A., Wincent, E., Pakkanen, H., Lehtivuori, H., Ihalainen, J., & Vehniäinen, E.-R. (2020). Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics. Science of the Total Environment, 745, 141031. https://doi.org/10.1016/j.scitotenv.2020.141031
JYU authors or editors
Publication details
All authors or editors: Rigaud, Cyril; Eriksson, Andreas; Krasnov, Aleksei; Wincent, Emma; Pakkanen, Hannu; Lehtivuori, Heli; Ihalainen, Janne; Vehniäinen, Eeva-Riikka
Journal or series: Science of the Total Environment
ISSN: 0048-9697
eISSN: 1879-1026
Publication year: 2020
Volume: 745
Pages range: 141031
Publisher: Elsevier BV
Publication country: Netherlands
Publication language: English
DOI: https://doi.org/10.1016/j.scitotenv.2020.141031
Publication open access: Not open
Publication channel open access:
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/71299
Abstract
Polycyclic aromatic hydrocarbons (PAHs) are contaminants of concern that impact every sphere of the environment. Despite several decades of research, their mechanisms of toxicity are still poorly understood. This study explores the mechanisms of cardiotoxicity of the three widespread model PAHs retene, pyrene and phenanthrene in the rainbow trout (Oncorhynchus mykiss) early life stages. Newly hatched larvae were exposed to each individual compound at sublethal doses causing no significant increase in the prevalence of deformities. Changes in the cardiac transcriptome were assessed after 1, 3, 7 and 14 days of exposure using custom Salmo salar microarrays. The highest number of differentially expressed genes was observed after 1 or 3 days of exposure, and retene was the most potent compound in that regard. Over-representation analyses suggested that genes related to cardiac ion channels, calcium homeostasis and muscle contraction (actin binding, troponin and myosin complexes) were especially targeted by retene. Pyrene was also able to alter similar myosin-related genes, but at a different timing and in an opposite direction, suggesting compound-specific mechanisms of toxicity. Pyrene and to a lesser extent phenanthrene were altering key genes linked to the respiratory electron transport chain and to oxygen and iron metabolism. Overall, phenanthrene was not very potent in inducing changes in the cardiac transcriptome despite being apparently metabolized at a slower rate than retene and pyrene. The present study shows that exposure to different PAHs during the first few days of the swim-up stage can alter the expression of key genes involved into the cardiac development and function, which could potentially affect negatively the fitness of the larvae in the long term.
Keywords: water systems; impurities; aromatic hydrocarbons; toxins; toxicity; biological effects; Salmoniformes; rainbow trout; ecotoxicology
Free keywords: aquatic toxicology; cardiotoxicity; polycyclic aromatic hydrocarbons (PAHs); transcriptomics
Contributing organizations
Related projects
- Integrated OMICS and imaging for a better understanding of ecotoxicological mechanisms - PAH developmental toxicity as an example
- Vehniäinen, Eeva-Riikka
- Research Council of Finland
- Integrated OMICS and imaging for a better understanding of ecotoxicological mechanisms
- Vehniäinen, Eeva-Riikka
- Research Council of Finland
- Integrated OMICS and imaging for a better understanding of ecotoxicological mechanisms - PAH developmental toxicity as an example (research costs)
- Vehniäinen, Eeva-Riikka
- Research Council of Finland
Ministry reporting: Yes
Reporting Year: 2020
JUFO rating: 2
- Cell and Molecular Biology (Department of Biological and Environmental Science BIOENV) SMB
- Nanoscience Center (Department of Physics PHYS, JYFL) (Faculty of Mathematics and Science) (Department of Chemistry CHEM) (Department of Biological and Environmental Science BIOENV) NSC
- Environmental Science (Department of Biological and Environmental Science BIOENV) YMP