A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics (2020)
Rigaud, C., Eriksson, A., Krasnov, A., Wincent, E., Pakkanen, H., Lehtivuori, H., Ihalainen, J., & Vehniäinen, E.-R. (2020). Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics. Science of the Total Environment, 745, 141031. https://doi.org/10.1016/j.scitotenv.2020.141031
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Rigaud, Cyril; Eriksson, Andreas; Krasnov, Aleksei; Wincent, Emma; Pakkanen, Hannu; Lehtivuori, Heli; Ihalainen, Janne; Vehniäinen, Eeva-Riikka
Lehti tai sarja: Science of the Total Environment
ISSN: 0048-9697
eISSN: 1879-1026
Julkaisuvuosi: 2020
Volyymi: 745
Artikkelin sivunumerot: 141031
Kustantaja: Elsevier BV
Julkaisumaa: Alankomaat
Julkaisun kieli: englanti
DOI: https://doi.org/10.1016/j.scitotenv.2020.141031
Julkaisun avoin saatavuus: Ei avoin
Julkaisukanavan avoin saatavuus:
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/71299
Tiivistelmä
Polycyclic aromatic hydrocarbons (PAHs) are contaminants of concern that impact every sphere of the environment. Despite several decades of research, their mechanisms of toxicity are still poorly understood. This study explores the mechanisms of cardiotoxicity of the three widespread model PAHs retene, pyrene and phenanthrene in the rainbow trout (Oncorhynchus mykiss) early life stages. Newly hatched larvae were exposed to each individual compound at sublethal doses causing no significant increase in the prevalence of deformities. Changes in the cardiac transcriptome were assessed after 1, 3, 7 and 14 days of exposure using custom Salmo salar microarrays. The highest number of differentially expressed genes was observed after 1 or 3 days of exposure, and retene was the most potent compound in that regard. Over-representation analyses suggested that genes related to cardiac ion channels, calcium homeostasis and muscle contraction (actin binding, troponin and myosin complexes) were especially targeted by retene. Pyrene was also able to alter similar myosin-related genes, but at a different timing and in an opposite direction, suggesting compound-specific mechanisms of toxicity. Pyrene and to a lesser extent phenanthrene were altering key genes linked to the respiratory electron transport chain and to oxygen and iron metabolism. Overall, phenanthrene was not very potent in inducing changes in the cardiac transcriptome despite being apparently metabolized at a slower rate than retene and pyrene. The present study shows that exposure to different PAHs during the first few days of the swim-up stage can alter the expression of key genes involved into the cardiac development and function, which could potentially affect negatively the fitness of the larvae in the long term.
YSO-asiasanat: vesistöt; epäpuhtaudet; aromaattiset hiilivedyt; toksiinit; myrkyllisyys; biologiset vaikutukset; lohikalat; kirjolohi; ekotoksikologia
Vapaat asiasanat: aquatic toxicology; cardiotoxicity; polycyclic aromatic hydrocarbons (PAHs); transcriptomics
Liittyvät organisaatiot
Hankkeet, joissa julkaisu on tehty
- Omiikat ja kuvantaminen ekotoksikologisten mekanismien tunnistamisessa - esimerkkinä PAH-yhdisteiden aiheuttama kehitystoksisuus
- Vehniäinen, Eeva-Riikka
- Suomen Akatemia
- Omniikat ja kuvantaminen ekotoksikologisten mekanismien tunnistamisessa
- Vehniäinen, Eeva-Riikka
- Suomen Akatemia
- Omiikat ja kuvantaminen ekotoksikologisten mekanismien tunnistamisessa - esimerkkinä PAH-yhdisteiden aiheuttama kehitystoksisuus (tutkimuskulut)
- Vehniäinen, Eeva-Riikka
- Suomen Akatemia
OKM-raportointi: Kyllä
Raportointivuosi: 2020
JUFO-taso: 2