A1 Journal article (refereed)
Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, Part 2 – Proteomics and metabolomics (2020)

Rigaud, C., Eriksson, A., Rokka, A., Skaugen, M., Lihavainen, J., Keinänen, M., Lehtivuori, H., & Vehniäinen, E.-R. (2020). Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, Part 2 – Proteomics and metabolomics. Science of the Total Environment, 746, Article 141161. https://doi.org/10.1016/j.scitotenv.2020.141161

JYU authors or editors

Rigaud, Cyril
Eriksson, Andreas
Lehtivuori, Heli
Vehniäinen, Eeva-Riikka

Publication details

All authors or editors: Rigaud, Cyril; Eriksson, Andreas; Rokka, Anne; Skaugen, Morten; Lihavainen, Jenna; Keinänen, Markku; Lehtivuori, Heli; Vehniäinen, Eeva-Riikka

Journal or series: Science of the Total Environment

ISSN: 0048-9697

eISSN: 1879-1026

Publication year: 2020

Volume: 746

Article number: 141161

Publisher: Elsevier BV

Publication country: Netherlands

Publication language: English

DOI: https://doi.org/10.1016/j.scitotenv.2020.141161

Publication open access: Not open

Publication channel open access:

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/71315

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are global contaminants of concern. Despite several decades of research, their mechanisms of toxicity are not very well understood. Early life stages of fish are particularly sensitive with the developing cardiac tissue being a main target of PAHs toxicity. The mechanisms of cardiotoxicity of the three widespread model polycyclic aromatic hydrocarbons (PAHs) retene, pyrene and phenanthrene were explored in rainbow trout (Oncorhyncus mykiss) early life stages. Newly hatched larvae were exposed to sublethal doses of each individual PAH causing no detectable morphometric alterations. Changes in the cardiac proteome and metabolome were assessed after 7 or 14 days of exposure to each PAH. Phase I and II enzymes regulated by the aryl hydrocarbon receptor were significantly induced by all PAHs, with retene being the most potent compound. Retene significantly altered the level of several proteins involved in key cardiac functions such as muscle contraction, cellular tight junctions or calcium homeostasis. Those findings were quite consistent with previous reports regarding the effects of retene on the cardiac transcriptome. Significant changes in proteins linked to iron and heme metabolism were observed following exposure to pyrene. While phenanthrene also altered the levels of several proteins in the cardiac tissue, no clear mechanisms or pathways could be highlighted. Due to high variability between samples, very few significant changes were detected in the cardiac metabolome overall. Slight but significant changes were still observed for pyrene and phenanthrene, suggesting possible effects on several energetic or signaling pathways. This study shows that early exposure to different PAHs can alter the expression of key proteins involved in the cardiac function, which could potentially affect negatively the fitness of the larvae and later of the juvenile fish.

Keywords: water systems; impurities; aromatic hydrocarbons; toxins; toxicity; biological effects; fishes; ecotoxicology; proteomics