A1 Journal article (refereed)
Interacting effects of simulated eutrophication, temperature increase, and microplastic exposure on Daphnia (2021)

Hiltunen, M., Vehniäinen, E.-R., & Kukkonen, J. V. K. (2021). Interacting effects of simulated eutrophication, temperature increase, and microplastic exposure on Daphnia. Environmental Research, 192, Article 110304. https://doi.org/10.1016/j.envres.2020.110304

JYU authors or editors

Publication details

All authors or editorsHiltunen, Minna; Vehniäinen, Eeva-Riikka; Kukkonen, Jussi V. K.

Journal or seriesEnvironmental Research



Publication year2021


Article number110304

PublisherElsevier BV

Publication countryUnited States

Publication languageEnglish


Publication open accessNot open

Publication channel open access

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/78390


The effects of multiple stressors are difficult to separate in field studies, and their interactions may be hard to predict if studied in isolation. We studied the effects of decreasing food quality (increase in cyanobacteria from 5 to 95% simulating eutrophication), temperature increase (by 3 °C), and microplastic exposure (1% of the diet) on survival, size, reproduction, and fatty acid composition of the model freshwater cladoceran Daphnia magna. We found that food quality was the major driver of Daphnia responses. When the amount of cyanobacteria increased from 5 to 95% of the diet, there was a drastic decrease in Daphnia survival (from 81 ± 15% to 24 ± 21%), juvenile size (from 1.8 ± 0.2 mm to 1.0 ± 0.1 mm), adult size (from 2.7 ± 0.1 mm to 1.1 ± 0.1 mm), and reproduction (from 13 ± 5 neonates per surviving adult to 0), but the decrease was not always linear. This was most likely due to lower availability of lipids, eicosapentaenoic acid (EPA), and sterols from the diet. Microplastic exposure did not affect Daphnia survival, size, or reproduction. Food quality had an interactive effect with temperature on fatty acid content of Daphnia. Total fatty acid content of Daphnia was almost 2-fold higher at 20 °C than at 23 °C when fed 50% cyanobacteria. This may have implications for higher trophic level consumers, such as fish, that depend on zooplankton for energy and essential lipids. Our findings suggest that as proportions of cyanobacteria increase, in tandem with water temperatures due to climate change, fish may encounter fewer and smaller Daphnia with lower lipid and EPA content.

KeywordsCladoceranutritionnutrients (animals and humans)qualitywater systemseutrophicationtemperaturelipidssterolsfatty acidssurvivalmicro-litterclimate changesplankton

Free keywordsCyanobacteria; fatty acids; climate change; food quality; Daphnia magna

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Ministry reportingYes

Reporting Year2021

JUFO rating2

Last updated on 2024-22-04 at 18:14