A1 Journal article (refereed)
Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats (2020)

Lensu, S., Pariyani, R., Mäkinen, E., Yang, B., Saleem, W., Munukka, E., Lehti, M., Driuchina, A., Lindén, J., Tiirola, M., Lahti, L., & Pekkala, S. (2020). Prebiotic Xylo-Oligosaccharides Ameliorate High-Fat-Diet-Induced Hepatic Steatosis in Rats. Nutrients, 12(11), Article 3225. https://doi.org/10.3390/nu12113225

JYU authors or editors

Publication details

All authors or editors: Lensu, Sanna; Pariyani, Raghunath; Mäkinen, Elina; Yang, Baoru; Saleem, Wisam; Munukka, Eveliina; Lehti, Maarit; Driuchina, Anastasiia; Lindén, Jere; Tiirola, Marja; et al.

Journal or series: Nutrients

eISSN: 2072-6643

Publication year: 2020

Publication date: 22/10/2020

Volume: 12

Issue number: 11

Article number: 3225

Publisher: MDPI AG

Publication country: Switzerland

Publication language: English

DOI: https://doi.org/10.3390/nu12113225

Publication open access: Openly available

Publication channel open access: Open Access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/72355

Abstract

Understanding the importance of the gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised the hope for therapeutic microbes. We have shown that high hepatic fat content associated with low abundance of Faecalibacterium prausnitzii in humans and, further, the administration of F. prausnitzii prevented NAFLD in mice. Here, we aimed at targeting F. prausnitzii by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD. First, the effect of XOS on F. prausnitzii growth was assessed in vitro. Then, XOS was supplemented or not with high (HFD, 60% of energy from fat) or low (LFD) fat diet for 12 weeks in Wistar rats (n = 10/group). XOS increased F. prausnitzii growth, having only a minor impact on the GM composition. When supplemented with HFD, XOS ameliorated hepatic steatosis. The underlying mechanisms involved enhanced hepatic β-oxidation and mitochondrial respiration. Nuclear magnetic resonance (1H-NMR) analysis of cecal metabolites showed that, compared to the HFD, the LFD group had a healthier cecal short-chain fatty acid profile and on the HFD, XOS reduced cecal isovalerate and tyrosine, metabolites previously linked to NAFLD. Cecal branched-chain fatty acids associated positively and butyrate negatively with hepatic triglycerides. In conclusion, XOS supplementation can ameliorate NAFLD by improving hepatic oxidative metabolism and affecting GM.

Keywords: gastrointestinal microbiota; prebiotics; metabolism; metabolic products; oligosaccharides; mitochondria; fatty liver

Free keywords: prebiotic; oligosaccharides; gut microbiota; fatty liver; metabolism; mitochondria

Contributing organizations

Related projects

Ministry reporting: Yes

VIRTA submission year: 2020

JUFO rating: 1