A1 Journal article (refereed)
Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes (2021)


Rissanen, A. J., Saarela, T., Jäntti, H., Buck, M., Peura, S., Aalto, S. L., Ojala, A., Pumpanen, J., Tiirola, M., Elvert, M., & Nykänen, H. (2021). Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes. Fems Microbiology Ecology, 97(2), Article fiaa252. https://doi.org/10.1093/femsec/fiaa252


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Publication details

All authors or editorsRissanen, Antti J.; Saarela, Taija; Jäntti, Helena; Buck, Moritz; Peura, Sari; Aalto, Sanni L.; Ojala, Anne; Pumpanen, Jukka; Tiirola, Marja; Elvert, Marcus; et al.

Journal or seriesFems Microbiology Ecology

ISSN0168-6496

eISSN1574-6941

Publication year2021

Volume97

Issue number2

Article numberfiaa252

PublisherOxford University Press

Publication countryUnited Kingdom

Publication languageEnglish

DOIhttps://doi.org/10.1093/femsec/fiaa252

Publication open accessOpenly available

Publication channel open accessPartially open access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/74055


Abstract

The vertical structuring of methanotrophic communities and its genetic controllers remain understudied in the water columns of oxygen-stratified lakes. Therefore, we used 16S rRNA gene sequencing to study the vertical stratification patterns of methanotrophs in two boreal lakes, Lake Kuivajärvi and Lake Lovojärvi. Furthermore, metagenomic analyses were done to assess the genomic characteristics of methanotrophs in Lovojärvi and a previously studied Lake Alinen Mustajärvi. The methanotroph communities were vertically structured along the oxygen gradient. Alphaproteobacterial methanotrophs preferred oxic water layers, while Methylococcales methanotrophs, consisting of putative novel genera and species, thrived especially at and below the oxic-anoxic interface and showed distinct depth variation patterns, which were not completely predictable by their taxonomic classification. Instead, genomic differences among Methylococcales methanotrophs explained their variable vertical depth patterns. Genes in COG categories L (Replication, recombination and repair) and S (Function unknown) were relatively high in metagenome-assembled-genomes representing Methylococcales thriving clearly below the oxic-anoxic interface, suggesting genetic adaptations for increased stress tolerance enabling living in the hypoxic/anoxic conditions. In contrast, genes in COG category N (Cell motility) were relatively high in metagenome-assembled-genomes of Methylococcales thriving at the oxic-anoxic interface, which suggests genetic adaptations for increased motility at the vertically fluctuating oxic-anoxic interface.


Keywordslakesmicrobiomestratificationoxygen depletionmethanegenomicsRNA

Free keywordsmethanotroph; Methylococcales; vertical structuring; 16S rRNA; metagenomics; genetic potential


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Ministry reportingYes

Reporting Year2021

JUFO rating1


Last updated on 2024-03-04 at 19:57