A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection (2021)


Xu, M., Perdomo, M. F., Mattola, S., Pyöriä, L., Toppinen, M., Qiu, J., Vihinen-Ranta, M., Hedman, K., Nokso-Koivisto, J., Aaltonen, L.-M., & Söderlund-Venermo, M. (2021). Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection. mBio, 12(1), Article e03132-20. https://doi.org/10.1128/mbio.03132-20


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Xu, Man; Perdomo, Maria Fernanda; Mattola, Salla; Pyöriä, Lari; Toppinen, Mari; Qiu, Jianming; Vihinen-Ranta, Maija; Hedman, Klaus; Nokso-Koivisto, Johanna; Aaltonen, Leena-Maija; et al.

Lehti tai sarja: mBio

ISSN: 2161-2129

eISSN: 2150-7511

Julkaisuvuosi: 2021

Volyymi: 12

Lehden numero: 1

Artikkelinumero: e03132-20

Kustantaja: American Society for Microbiology

Julkaisumaa: Yhdysvallat (USA)

Julkaisun kieli: englanti

DOI: https://doi.org/10.1128/mbio.03132-20

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/74021


Tiivistelmä

Human bocavirus 1 (HBoV1), a nonenveloped single-stranded DNA parvovirus, causes mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children. HBoV1 often persists in nasopharyngeal secretions for months, hampering diagnosis. It has also been shown to persist in pediatric palatine and adenoid tonsils, which suggests that lymphoid organs are reservoirs for virus spread; however, the tissue site and host cells remain unknown. Our aim was to determine, in healthy nonviremic children with preexisting HBoV1 immunity, the adenotonsillar persistence site(s), host cell types, and virus activity. We discovered that HBoV1 DNA persists in lymphoid germinal centers (GCs), but not in the corresponding tonsillar epithelium, and that the cell types harboring the virus are mainly naive, activated, and memory B cells and monocytes. Both viral DNA strands and both sides of the genome were detected, as well as infrequent mRNA. Moreover, we showed, in B-cell and monocyte cultures and ex vivo tonsillar B cells, that the cellular uptake of HBoV1 occurs via the Fc receptor (FcγRII) through antibody-dependent enhancement (ADE). This resulted in viral mRNA transcription, known to occur exclusively from double-stranded DNA in the nucleus, however, with no detectable productive replication. Confocal imaging with fluorescent virus-like particles moreover disclosed endocytosis. To which extent the active HBoV1 GC persistence has a role in chronic inflammation or B-cell maturation disturbances, and whether the virus can be reactivated, will be interesting topics for forthcoming studies.


YSO-asiasanat: virukset; parvovirukset; infektiot; vasta-aineet; nielurisa

Vapaat asiasanat: parvovirus; germinal center; tonsils; virus persistence; ADE; in situ hybridization


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointi: Kyllä

Raportointivuosi: 2021

Alustava JUFO-taso: 1


Viimeisin päivitys 2021-20-09 klo 16:11