A1 Journal article (refereed)
Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection (2021)

Xu, M., Perdomo, M. F., Mattola, S., Pyöriä, L., Toppinen, M., Qiu, J., Vihinen-Ranta, M., Hedman, K., Nokso-Koivisto, J., Aaltonen, L.-M., & Söderlund-Venermo, M. (2021). Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection. mBio, 12(1), Article e03132-20. https://doi.org/10.1128/mbio.03132-20

JYU authors or editors

Publication details

All authors or editors: Xu, Man; Perdomo, Maria Fernanda; Mattola, Salla; Pyöriä, Lari; Toppinen, Mari; Qiu, Jianming; Vihinen-Ranta, Maija; Hedman, Klaus; Nokso-Koivisto, Johanna; Aaltonen, Leena-Maija; et al.

Journal or series: mBio

ISSN: 2161-2129

eISSN: 2150-7511

Publication year: 2021

Publication date: 02/02/2021

Volume: 12

Issue number: 1

Article number: e03132-20

Publisher: American Society for Microbiology

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1128/mbio.03132-20

Publication open access: Openly available

Publication channel open access: Open Access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/74021

Abstract

Human bocavirus 1 (HBoV1), a nonenveloped single-stranded DNA parvovirus, causes mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children. HBoV1 often persists in nasopharyngeal secretions for months, hampering diagnosis. It has also been shown to persist in pediatric palatine and adenoid tonsils, which suggests that lymphoid organs are reservoirs for virus spread; however, the tissue site and host cells remain unknown. Our aim was to determine, in healthy nonviremic children with preexisting HBoV1 immunity, the adenotonsillar persistence site(s), host cell types, and virus activity. We discovered that HBoV1 DNA persists in lymphoid germinal centers (GCs), but not in the corresponding tonsillar epithelium, and that the cell types harboring the virus are mainly naive, activated, and memory B cells and monocytes. Both viral DNA strands and both sides of the genome were detected, as well as infrequent mRNA. Moreover, we showed, in B-cell and monocyte cultures and ex vivo tonsillar B cells, that the cellular uptake of HBoV1 occurs via the Fc receptor (FcγRII) through antibody-dependent enhancement (ADE). This resulted in viral mRNA transcription, known to occur exclusively from double-stranded DNA in the nucleus, however, with no detectable productive replication. Confocal imaging with fluorescent virus-like particles moreover disclosed endocytosis. To which extent the active HBoV1 GC persistence has a role in chronic inflammation or B-cell maturation disturbances, and whether the virus can be reactivated, will be interesting topics for forthcoming studies.

Keywords: viruses; parvoviruses; infections; antibodies; palatine tonsils

Free keywords: parvovirus; germinal center; tonsils; virus persistence; ADE; in situ hybridization

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Ministry reporting: Yes

Reporting Year: 2021

JUFO rating: 1