A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
A muramidase from Acremonium alcalophilum hydrolyse peptidoglycan found in the gastrointestinal tract of broiler chickens (2021)
Frederiksen, C. Ø., Cohn, M. T., Skov, L. K., Schmidt, E. G. W., Schnorr, K. M., Buskov, S., Leppänen, M., Maasilta, I., Perez-Calvo, E., Lopez-Ulibarri, R., & Klausen, M. (2021). A muramidase from Acremonium alcalophilum hydrolyse peptidoglycan found in the gastrointestinal tract of broiler chickens. Journal of Industrial Microbiology and Biotechnology, 48(1-2), Article kuab008. https://doi.org/10.1093/jimb/kuab008
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Frederiksen, Carsten Østergaard; Cohn, Marianne Thorup; Skov, Lars Kobberøe; Schmidt, Esben Gjerløff Wedebye; Schnorr, Kirk Matthew; Buskov, Steen; Leppänen, Miika; Maasilta, Ilari; Perez-Calvo, Estefania; Lopez-Ulibarri, Rual; et al.
Lehti tai sarja: Journal of Industrial Microbiology and Biotechnology
ISSN: 1367-5435
eISSN: 1476-5535
Julkaisuvuosi: 2021
Ilmestymispäivä: 04.02.2021
Volyymi: 48
Lehden numero: 1-2
Artikkelinumero: kuab008
Kustantaja: Oxford University Press (OUP)
Julkaisumaa: Britannia
Julkaisun kieli: englanti
DOI: https://doi.org/10.1093/jimb/kuab008
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/75308
Tiivistelmä
This study evaluates peptidoglycan hydrolysis by a microbial muramidase from the fungus Acremonium alcalophilum in vitro and in the gastrointestinal tract of broiler chickens. Peptidoglycan used for in vitro studies was derived from 5 gram-positive chicken gut isolate type strains. In vitro peptidoglycan hydrolysis was studied by three approaches: a) helium ion microscopy to identify visual phenotypes of hydrolysis b) reducing end assay to quantify solubilization of peptidoglycan fragments and c) mass spectroscopy to estimate relative abundances of soluble substrates and reaction products. Visual effects of peptidoglycan hydrolysis could be observed by helium ion microscopy and the increase in abundance of soluble peptidoglycan due to hydrolysis was quantified by a reducing end assay. Mass spectroscopy confirmed the release of hydrolysis products and identified muropeptides from the five different peptidoglycan sources. Peptidoglycan hydrolysis in chicken crop, jejunum and caecum samples was measured by quantifying the total and soluble muramic acid content. A significant increase in the proportion of the soluble muramic acid was observed in all three segments upon inclusion of the microbial muramidase in the diet.
YSO-asiasanat: antimikrobiset yhdisteet; entsyymit; hydrolyysi; homesienet; suolistomikrobisto; broilerit
Liittyvät organisaatiot
OKM-raportointi: Kyllä
Raportointivuosi: 2021
JUFO-taso: 1
