A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Rapid Quantification of Microalgae Growth with Hyperspectral Camera and Vegetation Indices (2021)


Salmi, P., Eskelinen, M. A., Leppänen, M. T., & Pölönen, I. (2021). Rapid Quantification of Microalgae Growth with Hyperspectral Camera and Vegetation Indices. Plants, 10(2), Article 341. https://doi.org/10.3390/plants10020341


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajatSalmi, Pauliina; Eskelinen, Matti A.; Leppänen, Matti T.; Pölönen, Ilkka

Lehti tai sarjaPlants

eISSN2223-7747

Julkaisuvuosi2021

Ilmestymispäivä10.02.2021

Volyymi10

Lehden numero2

Artikkelinumero341

KustantajaMDPI AG

JulkaisumaaSveitsi

Julkaisun kielienglanti

DOIhttps://doi.org/10.3390/plants10020341

Linkki tutkimusaineistoonhttps://jyx.jyu.fi/handle/123456789/71623

Julkaisun avoin saatavuusAvoimesti saatavilla

Julkaisukanavan avoin saatavuusKokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX)https://jyx.jyu.fi/handle/123456789/74237


Tiivistelmä

Spectral cameras are traditionally used in remote sensing of microalgae, but increasingly also in laboratory-scale applications, to study and monitor algae biomass in cultures. Practical and cost-efficient protocols for collecting and analyzing hyperspectral data are currently needed. The purpose of this study was to test a commercial, easy-to-use hyperspectral camera to monitor the growth of different algae strains in liquid samples. Indices calculated from wavebands from transmission imaging were compared against algae abundance and wet biomass obtained from an electronic cell counter, chlorophyll a concentration, and chlorophyll fluorescence. A ratio of selected wavebands containing near-infrared and red turned out to be a powerful index because it was simple to calculate and interpret, yet it yielded strong correlations to abundances strain-specifically (0.85 < r < 0.96, p < 0.001). When all the indices formulated as A/B, A/(A + B) or (A − B)/(A + B), where A and B were wavebands of the spectral camera, were scrutinized, good correlations were found amongst them for biomass of each strain (0.66 < r < 0.98, p < 0.001). Comparison of near-infrared/red index to chlorophyll a concentration demonstrated that small-celled strains had higher chlorophyll absorbance compared to strains with larger cells. The comparison of spectral imaging to chlorophyll fluorescence was done for one strain of green algae and yielded strong correlations (near-infrared/red, r = 0.97, p < 0.001). Consequently, we described a simple imaging setup and information extraction based on vegetation indices that could be used to monitor algae cultures.


YSO-asiasanatkasvillisuusmikrolevätmonitorointikaukokartoitusspektrikuvaus

Vapaat asiasanatmobile spectral camera; vegetation indices; monitoring; transmission imaging


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointiKyllä

VIRTA-lähetysvuosi2021

JUFO-taso1


Viimeisin päivitys 2024-12-10 klo 09:00