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Tuning protein adsorption on graphene surfaces via laser-induced oxidation (2021)

Sitsanidis, Efstratios D.; Schirmer, Johanna; Lampinen, Aku; Mentel, Kamila K.; Hiltunen, Vesa-Matti; Ruokolainen, Visa; Johansson, Andreas; Myllyperkiö, Pasi; Nissinen, Maija; Pettersson, Mika (2021). Tuning protein adsorption on graphene surfaces via laser-induced oxidation. Nanoscale Advances, Early online. DOI: 10.1039/d0na01028f

JYU-tekijät tai -toimittajat

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Sitsanidis, Efstratios D.; Schirmer, Johanna; Lampinen, Aku; Mentel, Kamila K.; Hiltunen, Vesa-Matti; Ruokolainen, Visa; Johansson, Andreas; Myllyperkiö, Pasi; Nissinen, Maija; Pettersson, Mika

Lehti tai sarja: Nanoscale Advances

eISSN: 2516-0230

Julkaisuvuosi: 2021

Volyymi: Early online

Kustantaja: Royal Society of Chemistry (RSC)

Julkaisumaa: Britannia

Julkaisun kieli: englanti

DOI: https://doi.org/10.1039/d0na01028f

Avoin saatavuus: Open access -julkaisukanavassa ilmestynyt julkaisu

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Julkaisun avoin saatavuus:

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/74473

Tiivistelmä

An approach for controlled protein immobilization on laser-induced two-photon (2P) oxidation patterned graphene oxide (GO) surfaces is described. Selected proteins, horseradish peroxidase (HRP) and biotinylated bovine serum albumin (b-BSA) were successfully immobilized on oxidized graphene surfaces, via non-covalent interactions, by immersion of graphene-coated microchips in the protein solution. The effects of laser pulse energy, irradiation time, protein concentration and duration of incubation on the topography of immobilized proteins and consequent defects upon the lattice of graphene were systemically studied by atomic force microscopy (AFM) and Raman spectroscopy. AFM and fluorescence microscopy confirmed the selective aggregation of protein molecules towards the irradiated areas. In addition, the attachment of b-BSA was detected by a reaction with fluorescently labelled avidin-fluorescein isothiocyanate (Av-FITC). In contrast to chemically oxidized graphene, laser-induced oxidation introduces the capability for localization on oxidized areas and tunability of the levels of oxidation, resulting in controlled guidance of proteins by light over graphene surfaces and progressing towards graphene microchips suitable for biomedical applications.

YSO-asiasanat: proteiinit; grafeeni; hapettuminen; lasertekniikka; atomivoimamikroskopia; fluoresenssimikroskopia; spektroskopia; mikrosirut

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