A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Tuning protein adsorption on graphene surfaces via laser-induced oxidation (2021)
Sitsanidis, Efstratios D.; Schirmer, Johanna; Lampinen, Aku; Mentel, Kamila K.; Hiltunen, Vesa-Matti; Ruokolainen, Visa; Johansson, Andreas; Myllyperkiö, Pasi; Nissinen, Maija; Pettersson, Mika (2021). Tuning protein adsorption on graphene surfaces via laser-induced oxidation. Nanoscale Advances, Early online. DOI: 10.1039/d0na01028f
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Sitsanidis, Efstratios D.; Schirmer, Johanna; Lampinen, Aku; Mentel, Kamila K.; Hiltunen, Vesa-Matti; Ruokolainen, Visa; Johansson, Andreas; Myllyperkiö, Pasi; Nissinen, Maija; Pettersson, Mika
Lehti tai sarja: Nanoscale Advances
eISSN: 2516-0230
Julkaisuvuosi: 2021
Volyymi: Early online
Kustantaja: Royal Society of Chemistry (RSC)
Julkaisumaa: Britannia
Julkaisun kieli: englanti
DOI: https://doi.org/10.1039/d0na01028f
Avoin saatavuus: Open access -julkaisukanavassa ilmestynyt julkaisu
Julkaisukanavan avoin saatavuus:
Julkaisun avoin saatavuus:
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/74473
Tiivistelmä
An approach for controlled protein immobilization on laser-induced two-photon (2P) oxidation patterned graphene oxide (GO) surfaces is described. Selected proteins, horseradish peroxidase (HRP) and biotinylated bovine serum albumin (b-BSA) were successfully immobilized on oxidized graphene surfaces, via non-covalent interactions, by immersion of graphene-coated microchips in the protein solution. The effects of laser pulse energy, irradiation time, protein concentration and duration of incubation on the topography of immobilized proteins and consequent defects upon the lattice of graphene were systemically studied by atomic force microscopy (AFM) and Raman spectroscopy. AFM and fluorescence microscopy confirmed the selective aggregation of protein molecules towards the irradiated areas. In addition, the attachment of b-BSA was detected by a reaction with fluorescently labelled avidin-fluorescein isothiocyanate (Av-FITC). In contrast to chemically oxidized graphene, laser-induced oxidation introduces the capability for localization on oxidized areas and tunability of the levels of oxidation, resulting in controlled guidance of proteins by light over graphene surfaces and progressing towards graphene microchips suitable for biomedical applications.
YSO-asiasanat: proteiinit; grafeeni; hapettuminen; lasertekniikka; atomivoimamikroskopia; fluoresenssimikroskopia; spektroskopia; mikrosirut
Liittyvät organisaatiot
Hankkeet, joissa julkaisu on tehty
- Yliopistojen profiloitumisen vahvistaminen kilpaillulla rahoituksella (Profi4). Profilointitoimet JYUssä 4. kierros. SA Profilointi 2018-2022
- Hämäläinen, Keijo
- Suomen Akatemia
OKM-raportointi: Kyllä
Alustava JUFO-taso: 1