A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton (2021)

Salmi, P., Mäki, A., Mikkonen, A., Pupponen, V.-M., Vuorio, K., & Tiirola, M. (2021). Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton. Boreal Environment Research, 26, 17-27. http://www.borenv.net/BER/archive/pdfs/ber26/ber26-017-027.pdf

JYU-tekijät tai -toimittajat

Salmi, Pauliina
Mäki, Anita
Mikkonen, Anu
Puupponen, Veli-Mikko
Tiirola, Marja

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Salmi, Pauliina; Mäki, Anita; Mikkonen, Anu; Pupponen, Veli-Mikko; Vuorio, Kristiina; Tiirola, Marja

Lehti tai sarja: Boreal Environment Research

ISSN: 1239-6095

eISSN: 1797-2469

Julkaisuvuosi: 2021

Volyymi: 26

Artikkelin sivunumerot: 17-27

Kustantaja: Suomen ympäristökeskus

Julkaisumaa: Suomi

Julkaisun kieli: englanti

Pysyvä verkko-osoite: http://www.borenv.net/BER/archive/pdfs/ber26/ber26-017-027.pdf

Linkki tutkimusaineistoon: http://urn.fi/URN:NBN:fi:jyu-201911084794

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/76018

Tiivistelmä

The smaller the phytoplankton, the greater effort is required to distinguish individual cells by optics-based methods. Flow cytometry is widely applied in marine picophytoplankton research, but in freshwater research its role has remained minor. We compared epifluorescence microscopy and flow cytometry in assessing the composition, abundance and cell sizes of autofluorescent picophytoplankton in epilimnia of 46 Finnish lakes. Phycocyaninrich picocyanobacteria were the most dominant. The two methods yielded comparable total picophytoplankton abundances, but the determination of cell sizes, and thus total biomasses, were on average an order of magnitude higher in the microscopy results. However, flow cytometry yielded higher cell sizes when applied on small-celled cultured algae. Our study demonstrated that both epifluorescence microscopy and flow cytometry are useful methods in assessing abundances of phycocyanin-rich and phycoerythrin-rich picocyanobacteria and eukaryotic picophytoplankton in lakes. However, accurate determination of cell size and biomass remain challenges for microscopy and especially for flow cytometry.

YSO-asiasanat: plankton; syanobakteerit; mikrolevät; mikroskopia; fluoresenssimikroskopia; biomassa (ekologia); laskentamallit