A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
A collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo (2021)

Civil, R., Brook, M. S., Elliott‐Sale, K. J., Santos, L., Varley, I., Lensu, S., Kainulainen, H., Koch, L. G., Britton, S. L., Wilkinson, D. J., Smith, K., Sale, C., & Atherton, P. J. (2021). A collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo. Physiological Reports, 9(10), Article e14799. https://doi.org/10.14814/phy2.14799

JYU-tekijät tai -toimittajat

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Civil, Rita; Brook, Matthew S.; Elliott‐Sale, Kirsty J.; Santos, Lívia; Varley, Ian; Lensu, Sanna; Kainulainen, Heikki; Koch, Lauren G.; Britton, Steven L.; Wilkinson, Daniel J.; et al.

Lehti tai sarja: Physiological Reports

ISSN: 2051-817X

eISSN: 2051-817X

Julkaisuvuosi: 2021

Volyymi: 9

Lehden numero: 10

Artikkelinumero: e14799

Kustantaja: John Wiley & Sons

Julkaisumaa: Britannia

Julkaisun kieli: englanti

DOI: https://doi.org/10.14814/phy2.14799

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/76082

Tiivistelmä

The development of safe and practical strategies to prevent weakening of bone tissue is vital, yet attempts to achieve this have been hindered by a lack of understanding of the short-term (days-weeks) physiology of bone collagen turnover. To address this, we have developed a method to quantify bone collagen synthesis in vivo, using deuterium oxide (D2O) tracer incorporation techniques combined with gas chromatography pyrolysis isotope-ratio mass spectrometry (GC-pyrolysis-IRMS). Forty-six male and female rats from a selectively bred model ingested D2O for 3 weeks. Femur diaphyses (FEM), tibia proximal (T-PRO), and distal (T-DIS) epiphyses-metaphyses and tibia mid-shaft diaphyses (T-MID) were obtained from all rats after necropsy. After demineralisation, collagen proteins were isolated and hydrolysed and collagen fractional synthetic rates (FSRs) determined by incorporation of deuterium into protein-bound alanine via GC-pyrolysis-IRMS. The collagen FSR for the FEM (0.131 ± 0.078%/day; 95% CI [0.106–0.156]) was greater than the FSR at T-MID (0.055 ± 0.049%/day; 95% CI [0.040–0.070]; p < 0.001). The T-PRO site had the highest FSR (0.203 ± 0.123%/day; 95% CI [0.166–0.241]) and T-DIS the lowest (0.027 ± 0.015%/day; 95% CI [0.022–0.031]). The three tibial sites exhibited different FSRs (p < 0.001). Herein, we have developed a sensitive method to quantify in vivo bone collagen synthesis and identified site-specific rates of synthesis, which could be applicable to studies of human bone collagen turnover.

YSO-asiasanat: luu; luukudokset; regeneraatio (biologia); kollageenit; biomarkkerit; isotooppianalyysi

Vapaat asiasanat: bone turnover; collagen synthesis; deuterium oxide; GC-pyrolysis-IRMS; stable isotopes

Liittyvät organisaatiot

OKM-raportointi: Kyllä

Raportointivuosi: 2021

JUFO-taso: 1