A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Laboratory phase‐contrast nanotomography of unstained Bombus terrestris compound eyes (2021)
Romell, J., Jie, V. W., Miettinen, A., Baird, E., & Hertz, H. M. (2021). Laboratory phase‐contrast nanotomography of unstained Bombus terrestris compound eyes. Journal of Microscopy, 283(1), 29-40. https://doi.org/10.1111/jmi.13005
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Romell, Jenny; Jie, Vun Wen; Miettinen, Arttu; Baird, Emily; Hertz, Hans M.
Lehti tai sarja: Journal of Microscopy
ISSN: 0022-2720
eISSN: 1365-2818
Julkaisuvuosi: 2021
Ilmestymispäivä: 08.05.2021
Volyymi: 283
Lehden numero: 1
Artikkelin sivunumerot: 29-40
Kustantaja: Wiley
Julkaisumaa: Britannia
Julkaisun kieli: englanti
DOI: https://doi.org/10.1111/jmi.13005
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/76513
Tiivistelmä
Imaging the visual systems of bumblebees and other pollinating insects may increase understanding of their dependence on specific habitats and how they will be affected by climate change. Current high-resolution imaging methods are either limited to two dimensions (light- and electron microscopy) or have limited access (synchrotron radiation x-ray tomography). For x-ray imaging, heavy metal stains are often used to increase contrast. Here, we present micron-resolution imaging of compound eyes of buff-tailed bumblebees (Bombus terrestris) using a table-top x-ray nanotomography (nano-CT) system. By propagation-based phase-contrast imaging, the use of stains was avoided and the microanatomy could more accurately be reconstructed than in samples stained with phosphotungstic acid or osmium tetroxide. The findings in the nano-CT images of the compound eye were confirmed by comparisons with light- and transmission electron microscopy of the same sample and finally, comparisons to synchrotron radiation tomography as well as to a commercial micro-CT system were done.
YSO-asiasanat: kuvantaminen; tietokonetomografia; kimalaiset; silmät
Vapaat asiasanat: bumblebee; compound eyes; laboratory nano-CT; phase contrast system; comparison
Liittyvät organisaatiot
OKM-raportointi: Kyllä
Raportointivuosi: 2021
JUFO-taso: 1
