A1 Journal article (refereed)
Laboratory phase‐contrast nanotomography of unstained Bombus terrestris compound eyes (2021)

Romell, J., Jie, V. W., Miettinen, A., Baird, E., & Hertz, H. M. (2021). Laboratory phase‐contrast nanotomography of unstained Bombus terrestris compound eyes. Journal of Microscopy, 283(1), 29-40. https://doi.org/10.1111/jmi.13005

JYU authors or editors

Publication details

All authors or editors: Romell, Jenny; Jie, Vun Wen; Miettinen, Arttu; Baird, Emily; Hertz, Hans M.

Journal or series: Journal of Microscopy

ISSN: 0022-2720

eISSN: 1365-2818

Publication year: 2021

Publication date: 08/05/2021

Volume: 283

Issue number: 1

Pages range: 29-40

Publisher: Wiley

Publication country: United Kingdom

Publication language: English

DOI: https://doi.org/10.1111/jmi.13005

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/76513

Abstract

Imaging the visual systems of bumblebees and other pollinating insects may increase understanding of their dependence on specific habitats and how they will be affected by climate change. Current high-resolution imaging methods are either limited to two dimensions (light- and electron microscopy) or have limited access (synchrotron radiation x-ray tomography). For x-ray imaging, heavy metal stains are often used to increase contrast. Here, we present micron-resolution imaging of compound eyes of buff-tailed bumblebees (Bombus terrestris) using a table-top x-ray nanotomography (nano-CT) system. By propagation-based phase-contrast imaging, the use of stains was avoided and the microanatomy could more accurately be reconstructed than in samples stained with phosphotungstic acid or osmium tetroxide. The findings in the nano-CT images of the compound eye were confirmed by comparisons with light- and transmission electron microscopy of the same sample and finally, comparisons to synchrotron radiation tomography as well as to a commercial micro-CT system were done.

Keywords: imaging; computed tomography; bumblebees; eyes

Free keywords: bumblebee; compound eyes; laboratory nano-CT; phase contrast system; comparison

Contributing organizations

Ministry reporting: Yes

Reporting Year: 2021

JUFO rating: 1