A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Bacteriophage Resistance Affects Flavobacterium columnare Virulence Partly via Mutations in Genes Related to Gliding Motility and Type IX Secretion System (2021)
Kunttu, H. M. T., Runtuvuori-Salmela, A., Sundell, K., Wiklund, T., Middelboe, M., Landor, L., Ashrafi, R., Hoikkala, V., & Sundberg, L.-R. (2021). Bacteriophage Resistance Affects Flavobacterium columnare Virulence Partly via Mutations in Genes Related to Gliding Motility and Type IX Secretion System. Applied and Environmental Microbiology, 87(16), e00812-21. https://doi.org/10.1128/AEM.00812-21
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Kunttu, Heidi M. T.; Runtuvuori-Salmela, Anniina; Sundell, Krister; Wiklund, Tom; Middelboe, Mathias; Landor, Lotta; Ashrafi, Roghaieh; Hoikkala, Ville; Sundberg, Lotta-Riina
Lehti tai sarja: Applied and Environmental Microbiology
ISSN: 0099-2240
eISSN: 1098-5336
Julkaisuvuosi: 2021
Ilmestymispäivä: 09.06.2021
Volyymi: 87
Lehden numero: 16
Artikkelin sivunumerot: e00812-21
Kustantaja: American Society for Microbiology
Julkaisumaa: Yhdysvallat (USA)
Julkaisun kieli: englanti
DOI: https://doi.org/10.1128/AEM.00812-21
Julkaisun avoin saatavuus: Ei avoin
Julkaisukanavan avoin saatavuus:
Rinnakkaistallenteen verkko-osoite (pre-print): https://www.biorxiv.org/content/10.1101/2020.10.02.323337v2
Tiivistelmä
Increasing problems with antibiotic resistance has directed interest towards phage therapy in the aquaculture industry. However, phage resistance evolving in target bacteria is considered a challenge. To investigate how phage resistance influences the fish pathogen Flavobacterium columnare, two wild-type bacterial isolates, FCO-F2 and FCO-F9, were exposed to phages (FCO-F2 to FCOV-F2, FCOV-F5 and FCOV-F25, and FCO-F9 to FCL-2, FCOV-F13 and FCOV-F45), and resulting phenotypic and genetic changes in bacteria were analyzed. Bacterial viability first decreased in the exposure cultures, but started to increase after 1-2 days, along with a change in colony morphology from original rhizoid to rough, leading to 98% prevalence of the rough morphotype. Twenty-four isolates (including four isolates from no-phage treatments) were further characterized for phage resistance, antibiotic susceptibility, motility, adhesion and biofilm formation, protease activity, whole genome sequencing and virulence in rainbow trout fry. The rough isolates arising in phage exposure were phage-resistant with low virulence, whereas rhizoid isolates maintained phage susceptibility and high virulence. Gliding motility and protease activity were also related to the phage susceptibility. Observed mutations in phage-resistant isolates were mostly located in genes coding for type IX secretion system, a component of the Bacteroidetes gliding motility machinery. However, not all phage-resistant isolates had mutations, indicating that phage resistance in F. columnare is a multifactorial process including both genetic mutations and changes in gene expression. Phage resistance may not, however, be a challenge for development of phage therapy against F. columnare infections, since phage resistance is associated with decrease in bacterial virulence.
YSO-asiasanat: bakteerit; bakteriofagit; fagiterapia; resistenssi; virulenssi; mutaatiot; kalataudit
Liittyvät organisaatiot
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- Sundberg, Lotta-Riina
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- Faagiteknologiaa taudinaiheuttajien torjuntaan kalanviljelyssä
- Sundberg, Lotta-Riina
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OKM-raportointi: Kyllä
Raportointivuosi: 2021
JUFO-taso: 2
- Akvaattiset tieteet (Bio- ja ympäristötieteiden laitos BIOENV) WET
- Solu- ja molekyylibiologia (Bio- ja ympäristötieteiden laitos BIOENV) SMB
- Ekologia ja evoluutiobiologia (Bio- ja ympäristötieteiden laitos BIOENV) EKO
- Nanoscience Center (Fysiikan laitos PHYS, JYFL) (Matemaattis-luonnontieteellinen tiedekunta) (Kemian laitos CHEM) (Bio- ja ympäristötieteiden laitos BIOENV) NSC