A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Multiple paths to cold tolerance : the role of environmental cues, morphological traits and the circadian clock gene vrille (2021)
Poikela, N., Tyukmaeva, V., Hoikkala, A., & Kankare, M. (2021). Multiple paths to cold tolerance : the role of environmental cues, morphological traits and the circadian clock gene vrille. BMC Ecology and Evolution, 21, Article 117. https://doi.org/10.1186/s12862-021-01849-y
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Poikela, Noora; Tyukmaeva, Venera; Hoikkala, Anneli; Kankare, Maaria
Lehti tai sarja: BMC Ecology and Evolution
eISSN: 2730-7182
Julkaisuvuosi: 2021
Ilmestymispäivä: 10.06.2021
Volyymi: 21
Artikkelinumero: 117
Kustantaja: Biomed Central
Julkaisumaa: Britannia
Julkaisun kieli: englanti
DOI: https://doi.org/10.1186/s12862-021-01849-y
Linkki tutkimusaineistoon: https://doi.org/10.5061/dryad.98sf7m0fv
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/78376
Tiivistelmä
Tracing the association between insect cold tolerance and latitudinally and locally varying environmental conditions, as well as key morphological traits and molecular mechanisms, is essential for understanding the processes involved in adaptation. We explored these issues in two closely-related species, Drosophila montana and Drosophila flavomontana, originating from diverse climatic locations across several latitudes on the coastal and mountainous regions of North America. We also investigated the association between sequence variation in one of the key circadian clock genes, vrille, and cold tolerance in both species. Finally, we studied the impact of vrille on fly cold tolerance and cold acclimation ability by silencing it with RNA interference in D. montana.
Results
We performed a principal component analysis (PCA) on variables representing bioclimatic conditions on the study sites and used latitude as a proxy of photoperiod. PC1 separated the mountainous continental sites from the coastal ones based on temperature variability and precipitation, while PC2 arranged the sites based on summer and annual mean temperatures. Cold tolerance tests showed D. montana to be more cold-tolerant than D. flavomontana and chill coma resistance (CTmin) of this species showed an association with PC2. Chill coma recovery time (CCRT) of both species improved towards northern latitudes, and in D. flavomontana this trait was also associated with PC1. D. flavomontana flies were darkest in the coast and in the northern mountainous populations, but coloration showed no linkage with cold tolerance. Body size decreased towards cold environments in both species, but only within D. montana populations largest flies showed fastest recovery from cold. Finally, both the sequence analysis and RNAi study on vrille suggested this gene to play an essential role in D. montana cold resistance and acclimation, but not in recovery time.
Conclusions
Our study demonstrates the complexity of insect cold tolerance and emphasizes the need to trace its association with multiple environmental variables and morphological traits to identify potential agents of natural selection. It also shows that a circadian clock gene vrille is essential both for short- and long-term cold acclimation, potentially elucidating the connection between circadian clock system and cold tolerance.
YSO-asiasanat: sopeutuminen; luonnonvalinta; kylmänkestävyys; morfologia; ympäristötekijät; vuorokausirytmi; geenit; RNA-interferenssi; mahlakärpäset
Vapaat asiasanat: CTmin; CCRT; body colour; body weight; latitude; bioclimatic variables; RNA interference (RNAi); Drosophila montana; Drosophila flavomontana
Liittyvät organisaatiot
OKM-raportointi: Kyllä
VIRTA-lähetysvuosi: 2021
JUFO-taso: 2
