A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Probing the Conformational States of a pH-Sensitive DNA Origami Zipper via Label-Free Electrochemical Methods (2021)

Williamson, P., Ijäs, H., Shen, B., Corrigan, D. K., & Linko, V. (2021). Probing the Conformational States of a pH-Sensitive DNA Origami Zipper via Label-Free Electrochemical Methods. Langmuir, 37(25), 7801-7809. https://doi.org/10.1021/acs.langmuir.1c01110

JYU-tekijät tai -toimittajat

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Williamson, Paul; Ijäs, Heini; Shen, Boxuan; Corrigan, Damion K.; Linko, Veikko

Lehti tai sarja: Langmuir

ISSN: 0743-7463

eISSN: 1520-5827

Julkaisuvuosi: 2021

Ilmestymispäivä: 15.06.2021

Volyymi: 37

Lehden numero: 25

Artikkelin sivunumerot: 7801-7809

Kustantaja: American Chemical Society

Julkaisumaa: Yhdysvallat (USA)

Julkaisun kieli: englanti

DOI: https://doi.org/10.1021/acs.langmuir.1c01110

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/76954

Tiivistelmä

DNA origami structures represent an exciting class of materials for use in a wide range of biotechnological applications. This study reports the design, production, and characterization of a DNA origami “zipper” structure, which contains nine pH-responsive DNA locks. Each lock consists of two parts that are attached to the zipper’s opposite arms: a DNA hairpin and a single-stranded DNA that are able to form a DNA triplex through Hoogsteen base pairing. The sequences of the locks were selected in a way that the zipper adopted a closed configuration at pH 6.5 and an open state at pH 8.0 (transition pKa 7.6). By adding thiol groups, it was possible to immobilize the zipper structure onto gold surfaces. The immobilization process was characterized electrochemically to confirm successful adsorption of the zipper. The open and closed states were then probed using differential pulse voltammetry and electrochemical impedance spectroscopy with solution-based redox agents. It was found that after immobilization, the open or closed state of the zipper in different pH regimes could be determined by electrochemical interrogation. These findings pave the way for development of DNA origami-based pH monitoring and other pH-responsive sensing and release strategies for zipper-functionalized gold surfaces.

YSO-asiasanat: DNA; nanorakenteet; nanobiotekniikka; kulta; sähkökemia; adsorptio

Liittyvät organisaatiot

Hankkeet, joissa julkaisu on tehty

OKM-raportointi: Kyllä

Raportointivuosi: 2021

JUFO-taso: 2