A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Higher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation (2021)


Lautaoja, J. H., O'Connell, T., Mäntyselkä, S., Peräkylä, J., Kainulainen, H., Pekkala, S., Permi, P., & Hulmi, J. J. (2021). Higher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation. American Journal of Physiology : Endocrinology and Metabolism, 321(2), E229-E245. https://doi.org/10.1152/ajpendo.00133.2021


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Lautaoja, Juulia H.; O'Connell, Thomas; Mäntyselkä, Sakari; Peräkylä, Juuli; Kainulainen, Heikki; Pekkala, Satu; Permi, Perttu; Hulmi, Juha J.

Lehti tai sarja: American Journal of Physiology : Endocrinology and Metabolism

ISSN: 0193-1849

eISSN: 1522-1555

Julkaisuvuosi: 2021

Ilmestymispäivä: 28.06.2021

Volyymi: 321

Lehden numero: 2

Artikkelin sivunumerot: E229-E245

Kustantaja: American Physiological Society

Julkaisumaa: Yhdysvallat (USA)

Julkaisun kieli: englanti

DOI: https://doi.org/10.1152/ajpendo.00133.2021

Julkaisun avoin saatavuus: Ei avoin

Julkaisukanavan avoin saatavuus:

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/77055


Tiivistelmä

The application of exercise-like electrical pulse simulation (EL-EPS) has become a widely used exercise mimetic in vitro. EL-EPS produces similar physiological responses as in vivo exercise, while less is known about the detailed metabolic effects. Routinely the C2C12 myotubes are cultured in high glucose medium (4.5 g/l), which may alter EL-EPS responses. In this study, we evaluate the metabolic effects of EL-EPS under the high and low glucose (1.0 g/l) conditions to understand how substrate availability affects the myotube response to EL-EPS.The C2C12 myotube, media and cell-free media metabolites were analyzed using untargeted nuclear magnetic resonance (NMR)-based metabolomics. Further, translational and metabolic changes and possible exerkine effects were analyzed. EL-EPS enhanced substrate utilization as well as production and secretion of lactate, acetate, 3-hydroxybutyrate and branched chain fatty acids (BCFAs). The increase in BCFAs correlated with branched chain amino acids (BCAAs) and BCFAs were strongly decreased when myotubes were cultured without BCAAs suggesting the action of acyl-CoA thioesterases on BCAA catabolites. Notably, not all EL-EPS responses were augmented by high glucose because EL-EPS increased phosphorylated c-Jun N-terminal kinase and interleukin-6 secretion independent of glucose availability. Administration of acetate and EL-EPS conditioned media on HepG2 hepatocytes had no adverse effects on lipolysis or triacylglycerol content.Our results demonstrate that unlike in cell-free media, the C2C12 myotube and media metabolites were affected by EL-EPS, particularly under high glucose condition suggesting that media composition should be considered in future EL-EPS studies. Further, acetate and BCFAs were identified as putative exerkines warranting more research.


YSO-asiasanat: lihassolut; solufysiologia; aineenvaihdunta; in vitro -menetelmä; glukoosi; aineenvaihduntatuotteet; laktaatit; asetaatit; rasvahapot

Vapaat asiasanat: acetate; exerkine; metabolomics; skeletal muscle; branched chain fatty acids


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointi: Kyllä

Raportointivuosi: 2021

JUFO-taso: 2


Viimeisin päivitys 2022-14-09 klo 12:16