A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Genetic diversity and phenotypic characterization of Iodobacter limnosediminis associated with skin lesions in freshwater fish (2021)


Korkea‐aho, T. L., Viljamaa‐Dirks, S., Heinikainen, S., Kuronen, H., & Tiirola, M. (2021). Genetic diversity and phenotypic characterization of Iodobacter limnosediminis associated with skin lesions in freshwater fish. Journal of Fish Diseases, 44(11), 1711-1724. https://doi.org/10.1111/jfd.13490


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Korkea‐aho, Tiina Liisa; Viljamaa‐Dirks, Satu; Heinikainen, Sirpa; Kuronen, Henry; Tiirola, Marja

Lehti tai sarja: Journal of Fish Diseases

ISSN: 0140-7775

eISSN: 1365-2761

Julkaisuvuosi: 2021

Volyymi: 44

Lehden numero: 11

Artikkelin sivunumerot: 1711-1724

Kustantaja: Wiley-Blackwell

Julkaisumaa: Britannia

Julkaisun kieli: englanti

DOI: https://doi.org/10.1111/jfd.13490

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/77059


Tiivistelmä

The relatively unknown genus Iodobacter sp. has been repeatedly isolated from skin ulcers and saprolegniosis on freshwater fish in Finland, especially farmed salmonids. Genetic characterization verified that all 23 bacterial isolates studied here belonged to the species Iodobacter limnosediminis, previously undescribed from the fish microbiota. Whole-genome pulsed-field gel electrophoresis revealed variability between the I. limnosediminis strains, suggesting that they were most likely of environmental origin. Two I. limnosediminis strains caused lesions in 27%–53% of brown trout (Salmo trutta) injected intramuscularly (p ≤ .05). The lesions represented moderate to severe tissue damage, but for most fish, the tissues had been repaired by the end of the experiment through the accumulation of fibrocytes and macrophages at the site of the lesion. I. limnosediminis was reisolated from some lesions and/or internal organs. Phenotypically and biochemically, I. limnosediminis resembles several common bacterial species found in the aquatic environment, as it grows well on several media as whitish medium-sized colonies, is Gram negative and rod-shaped. Here, we characterized I. limnosediminis strains with several methods, including MALDI-TOF. This characterization will help in further investigations into the occurrence and possible involvement of I. limnosediminis in skin lesions of freshwater fish.


YSO-asiasanat: kalat; makea vesi; mikrobit; bakteeritaudit; kudokset

Vapaat asiasanat: freshwater fish; Iodobacter limnosediminis; skin lesion


Liittyvät organisaatiot


OKM-raportointi: Kyllä

Alustava JUFO-taso: 1


Viimeisin päivitys 2021-22-10 klo 10:51