A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout (2021)

Kunttu, H. M. T., Runtuvuori-Salmela, A., Middelboe, M., Clark, J., & Sundberg, L.-R. (2021). Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout. Antibiotics, 10(8), Article 914. https://doi.org/10.3390/antibiotics10080914

JYU-tekijät tai -toimittajat

Kunttu, Heidi
Runtuvuori-Salmela, Anniina
Sundberg, Lotta-Riina

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Kunttu, Heidi M. T.; Runtuvuori-Salmela, Anniina; Middelboe, Mathias; Clark, Jason; Sundberg, Lotta-Riina

Lehti tai sarja: Antibiotics

eISSN: 2079-6382

Julkaisuvuosi: 2021

Ilmestymispäivä: 27.07.2021

Volyymi: 10

Lehden numero: 8

Artikkelinumero: 914

Kustantaja: MDPI AG

Julkaisumaa: Sveitsi

Julkaisun kieli: englanti

DOI: https://doi.org/10.3390/antibiotics10080914

Linkki tutkimusaineistoon: http://urn.fi/URN:NBN:fi:jyu-202109104846

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/78465

Lisätietoja: This article belongs to the Special Issue Phages and the Evolution of Bacterial Pathogenicity.

Tiivistelmä

Viruses of bacteria, bacteriophages, specifically infect their bacterial hosts with minimal effects on the surrounding microbiota. They have the potential to be used in the prevention and treatment of bacterial infections, including in the field of food production. In aquaculture settings, disease-causing bacteria are often transmitted through the water body, providing several applications for phage-based targeting of pathogens, in the rearing environment, and in the fish. We tested delivery of phages by different methods (via baths, in phage-coated material, and via oral delivery in feed) to prevent and treat Flavobacterium columnare infections in rainbow trout fry using three phages (FCOV-S1, FCOV-F2, and FCL-2) and their hosts (FCO-S1, FCO-F2, and B185, respectively). Bath treatments given before bacterial infection and at the onset of the disease symptoms were the most efficient way to prevent F. columnare infections in rainbow trout, possibly due to the external nature of the disease. In a flow-through system, the presence of phage-coated plastic sheets delayed the onset of the disease. The oral administration of phages first increased disease progression, although total mortality was lower at the end of the experiment. When analysed for shelf-life, phage titers remained highest when maintained in bacterial culture media and in sterile lake water. Our results show that successful phage therapy treatment in the aquaculture setting requires optimisation of phage delivery methods in vivo.

YSO-asiasanat: vesiviljely (kalatalous); bakteerit; bakteriofagit; bakteeritaudit; hoitomenetelmät; fagiterapia; lohikalat

Vapaat asiasanat: aquaculture; bacteriophage; bacterial infection; columnaris disease; Flavobacterium columnare; phage therapy; phage delivery; rainbow trout; treatment; virulence

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