A1 Journal article (refereed)
Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A (2021)


Thapa, C., Roivas, P., Haataja, T., Permi, P., & Pentikäinen, U. (2021). Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A. FEBS Journal, Early online. https://doi.org/10.1111/febs.16150


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Publication details

All authors or editors: Thapa, Chandan; Roivas, Pekka; Haataja, Tatu; Permi, Perttu; Pentikäinen, Ulla

Journal or series: FEBS Journal

ISSN: 1742-464X

eISSN: 1742-4658

Publication year: 2021

Volume: Early online

Publisher: Wiley-Blackwell

Publication country: United Kingdom

Publication language: English

DOI: https://doi.org/10.1111/febs.16150

Publication open access: Openly available

Publication channel open access: Partially open access channel


Abstract

The vast diversity of protein phosphatase 2A (PP2A) holoenzyme composition ensures its multi-faceted role in the regulation of cellular growth and signal transduction. In several pathological conditions, such as cancer, PP2A is inhibited by endogenous inhibitor proteins. Several PP2A inhibitor proteins have been identified, one of which is α-endosulfine (ENSA). ENSA inhibits PP2A activity when it is phosphorylated at Ser67 by Greatwall (Gwl) kinase. The role of ENSA in PP2A inhibition is rather well characterized, but knowledge of the mechanism of inhibition is scarce. In this study, we have performed comprehensive structural characterization of ENSA, and its interaction with PP2A A- and various B56-subunit isoforms by combining NMR spectroscopy, SAXS and interaction assays. The results clearly indicate that ENSA is an intrinsically disordered protein containing three transient α-helical structures. ENSA was observed to interact PP2A mainly via A-subunit, as the affinity with the A-subunit is significantly stronger than with any of the B56-subunits. Based on our results, it seems that ENSA follows the dock-and-coalesce mechanism in associating with PP2A A-subunit. Taken together, our results provide an essential structural and molecular framework to understanding molecular bases of ENSA mediated PP2A inhibition, which is crucial for the development of new therapies for diseases linked to PP2A inhibition.


Keywords: proteins; cell signaling; inhibitors; NMR spectroscopy

Free keywords: DPs; PP2A; PP2A inhibitor protein; ENSA; NMR; SAXS


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Last updated on 2021-16-09 at 09:17