A1 Journal article (refereed)
Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes (2022)


Fachada, V., Rahkila, P., Fachada, N., Turpeinen, T., Kujala, U. M., & Kainulainen, H. (2022). Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes. Acta Histochemica, 124(3), Article 151869. https://doi.org/10.1016/j.acthis.2022.151869


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Publication details

All authors or editorsFachada, Vasco; Rahkila, Paavo; Fachada, Nuno; Turpeinen, Tuomas; Kujala, Urho M.; Kainulainen, Heikki

Journal or seriesActa Histochemica

ISSN0065-1281

eISSN1618-0372

Publication year2022

Publication date24/02/2022

Volume124

Issue number3

Article number151869

PublisherElsevier GmbH.

Publication countryGermany

Publication languageEnglish

DOIhttps://doi.org/10.1016/j.acthis.2022.151869

Publication open accessOpenly available

Publication channel open accessPartially open access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/80495


Abstract

Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.


Keywordsmuscleslipidsproteinsinsulin resistanceadult-onset diabetes

Free keywordslipid droplets; PLIN5; Type II diabetes; skeletal muscle; insulin resistance; fiber type


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Ministry reportingYes

Reporting Year2022

JUFO rating1


Last updated on 2024-03-04 at 18:35