A1 Journal article (refereed)
Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes (2022)

Fachada, V., Rahkila, P., Fachada, N., Turpeinen, T., Kujala, U. M., & Kainulainen, H. (2022). Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes. Acta Histochemica, 124(3), Article 151869. https://doi.org/10.1016/j.acthis.2022.151869

JYU authors or editors

Publication details

All authors or editors: Fachada, Vasco; Rahkila, Paavo; Fachada, Nuno; Turpeinen, Tuomas; Kujala, Urho M.; Kainulainen, Heikki

Journal or series: Acta Histochemica

ISSN: 0065-1281

eISSN: 1618-0372

Publication year: 2022

Publication date: 24/02/2022

Volume: 124

Issue number: 3

Article number: 151869

Publisher: Elsevier GmbH.

Publication country: Germany

Publication language: English

DOI: https://doi.org/10.1016/j.acthis.2022.151869

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/80495

Abstract

Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.

Keywords: muscles; lipids; proteins; insulin resistance; adult-onset diabetes

Free keywords: lipid droplets; PLIN5; Type II diabetes; skeletal muscle; insulin resistance; fiber type

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Ministry reporting: Yes

VIRTA submission year: 2022

JUFO rating: 1